Gwen Tindula1, Susan K Murphy2, Carole Grenier2, Zhiqing Huang2, Karen Huen1, Maria Escudero-Fung1, Asa Bradman1, Brenda Eskenazi1, Cathrine Hoyo2,3, Nina Holland1. 1. Center for Environmental Research & Children's Health (CERCH), School of Public Health, University of California, Berkeley, CA 94720, USA. 2. Epigenetics Research Laboratory, Department of Obstetrics & Gynecology, Duke University Medical Center, Durham, NC 27708, USA. 3. Department of Biological Sciences, Center for Human Health & the Environment, North Carolina State University (NCSU), Raleigh, NC 27606, USA.
Abstract
AIM: Imprinted genes exhibit expression in a parent-of-origin-dependent manner and are critical for child development. Recent limited evidence suggests that prenatal exposure to phthalates, ubiquitous endocrine disruptors, can affect their epigenetic dysregulation. MATERIALS & METHODS: We quantified DNA methylation of nine imprinted gene differentially methylated regions by pyrosequencing in 296 cord blood DNA samples in a Mexican-American cohort. Fetal exposure was estimated by phthalate metabolite concentrations in maternal urine samples during pregnancy. RESULTS: Several differentially methylated regions of imprinted genes were associated with high molecular weight phthalates. The most consistent, positive, and false discovery rate significant associations were observed for MEG3. CONCLUSION: Phthalate exposure in utero may affect methylation status of imprinted genes in newborn children.
AIM: Imprinted genes exhibit expression in a parent-of-origin-dependent manner and are critical for child development. Recent limited evidence suggests that prenatal exposure to phthalates, ubiquitous endocrine disruptors, can affect their epigenetic dysregulation. MATERIALS & METHODS: We quantified DNA methylation of nine imprinted gene differentially methylated regions by pyrosequencing in 296 cord blood DNA samples in a Mexican-American cohort. Fetal exposure was estimated by phthalate metabolite concentrations in maternal urine samples during pregnancy. RESULTS: Several differentially methylated regions of imprinted genes were associated with high molecular weight phthalates. The most consistent, positive, and false discovery rate significant associations were observed for MEG3. CONCLUSION:Phthalate exposure in utero may affect methylation status of imprinted genes in newborn children.
Entities:
Keywords:
DNA methylation; Mexican–Americans; cord blood; differentially methylated region; endocrine disruptors; imprinted genes; in utero exposure; newborns; phthalates; pyrosequencing
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