| Literature DB >> 29956273 |
Shi-Qiong Xu1, Simone Buraschi2, Ryuta Tanimoto1, Manuela Stefanello1, Antonino Belfiore3, Renato V Iozzo2, Andrea Morrione4.
Abstract
Progranulin has emerged in recent years as an important regulator of various biological functions including cell proliferation, wound healing, motility, and protection from apoptosis. Progranulin is also critical for transformation as established in several cancer models.Progranulin biological responses elicit through the activation of the Akt and MAPK pathways, which are critical for progranulin downstream signaling.In this chapter various experimental approaches aiming at detecting progranulin-mediated Akt and MAPK activation will be discussed.Entities:
Keywords: Akt; Confocal analysis; Electrophoresis; Immunofluorescence; MAP kinase; Nitrocellulose membrane; PathScan; Progranulin; SDS PAGE
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Year: 2018 PMID： 29956273 PMCID： PMC9186102 DOI： 10.1007/978-1-4939-8559-3_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745