Literature DB >> 29956253

Methods for Atomic Force Microscopy of Biological and Living Specimens.

Simone Dinarelli1, Marco Girasole1, Giovanni Longo2.   

Abstract

Two main precautions must be taken into account to obtain high-resolution morphological and nanomechanical characterization of biological specimens with an atomic force microscope: the tip-sample interaction and the sample-substrate adhesion. In this chapter we discuss the necessary steps for a correct preparation of three types of biological samples: erythrocytes, bacteria, and osteoblasts. The main goal is to deliver reproducible protocols to produce good cellular adhesion and minimizing the morphological alterations of the specimens.

Keywords:  Atomic force microscopy; Bacteria; Living cells; Nanomechanical information; Topography; Young’s modulus

Mesh：

Year:  2018        PMID： 29956253     DOI： 10.1007/978-1-4939-8591-3_31

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

Review 1.  A Review of the Effect of a Nanostructured Thin Film Formed by Titanium Carbide and Titanium Oxides Clustered around Carbon in Graphitic Form on Osseointegration.

Authors:  Roberto Scandurra; Anna Scotto d'Abusco; Giovanni Longo
Journal:  Nanomaterials (Basel)       Date:  2020-06-24       Impact factor: 5.076

2.  A new tool to determine the cellular metabolic landscape: nanotechnology to the study of Friedreich's ataxia.

Authors:  Tommaso Vannocci; Simone Dinarelli; Marco Girasole; Annalisa Pastore; Giovanni Longo
Journal:  Sci Rep       Date:  2019-12-17       Impact factor: 4.379

3.  Identification of Oxidative Stress in Red Blood Cells with Nanoscale Chemical Resolution by Infrared Nanospectroscopy.

Authors:  Francesco S Ruggeri; Curtis Marcott; Simone Dinarelli; Giovanni Longo; Marco Girasole; Giovanni Dietler; Tuomas P J Knowles
Journal:  Int J Mol Sci       Date:  2018-08-30       Impact factor: 5.923
  3 in total

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