Literature DB >> 2995271

Immunoblotting and enzyme-linked immunosorbent assay analysis of serological responses in patients infected with herpes simplex virus types 1 and 2.

M Lehtinen, V Koivisto, T Lehtinen, J Paavonen, P Leinikki.   

Abstract

Serological responses to soluble membrane and cytoplasmic antigens specified by herpes simplex type 1 (HSV1) and 2 (HSV2) were studied by immunoblotting and by enzyme-linked immunosorbent assay (ELISA). In the immunoblotting test, polypeptides migrating like the HSV1-specified glycoprotein C showed type-specific reactivity but could not always be detected. The immunoblotting and ELISA results were in agreement when antibody responses to HSV1- and HSV2-specified antigens were compared, and they allowed the identification of patients with HSV2 and/or HSV1 antibodies.

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Year:  1985        PMID： 2995271     DOI： 10.1159/000149614

Source DB:  PubMed          Journal:  Intervirology        ISSN： 0300-5526            Impact factor:   1.763


  7 in total

1.  Evaluation of three glycoprotein G2-based enzyme immunoassays for detection of antibodies to herpes simplex virus type 2 in human sera.

Authors:  A M Eis-Hübinger; M Däumer; B Matz; K E Schneweis
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

2.  Locations of herpes simplex virus type 2 glycoprotein B epitopes recognized by human serum immunoglobulin G antibodies.

Authors:  D E Goade; R Bell; T Yamada; G J Mertz; S Jenison
Journal:  J Virol       Date:  1996-05       Impact factor: 5.103

3.  Serological evaluation of herpes simplex virus type 1 and type 2 infections in pregnancy.

Authors:  M Arvaja; M Lehtinen; P Koskela; M Lappalainen; J Paavonen; T Vesikari
Journal:  Sex Transm Infect       Date:  1999-06       Impact factor: 3.519

4.  Seroreactive recombinant herpes simplex virus type 2-specific glycoprotein G.

Authors:  D L Parkes; C M Smith; J M Rose; J Brandis; S R Coates
Journal:  J Clin Microbiol       Date:  1991-04       Impact factor: 5.948

5.  Comparison of Western blot (immunoblot) and glycoprotein G-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera.

Authors:  R L Ashley; J Militoni; F Lee; A Nahmias; L Corey
Journal:  J Clin Microbiol       Date:  1988-04       Impact factor: 5.948

6.  Antibody activity to herpes simplex virus in mouse Ig classes and IgG subclasses.

Authors:  R R McKendall; W Woo
Journal:  Arch Virol       Date:  1988       Impact factor: 2.574

Review 7.  Immunoblotting and dot blotting.

Authors:  D I Stott
Journal:  J Immunol Methods       Date:  1989-05-12       Impact factor: 2.303
  7 in total

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