BACKGROUND: Historically, B cells have been considered as positive regulators of humoral immune responses. Specific B-cell subsets, however, negatively regulate immune responses and are termed "regulatory B cells" (Bregs). Recently, Bregs have been linked to not only inflammatory and autoimmune diseases, but also malignancies via suppressing anti-tumour immunity. OBJECTIVES: To investigate the involvement of Bregs in advanced mycosis fungoides (MF). MATERIALS & METHODS: The frequency of CD19+CD24hiCD27+ memory B cells and CD19+CD24hiCD38hi transitional B cells (which enrich IL-10-producing Bregs) was examined in peripheral blood from patients with advanced MF (n = 11) and healthy controls (n = 9) by flow cytometry. The frequency of IL-10-producing Bregs was also measured by flow cytometry. The correlation between frequency or number of B-cell subsets and disease severity markers was also analysed. RESULTS: The frequency of CD19+CD24hiCD27+ B cells, CD19+CD24hiCD38hi B cells, and IL-10-producing B cells was decreased in peripheral blood of advanced MF patients. The frequency and number of these B-cell subsets inversely correlated with serum soluble IL-2 receptor and serum lactate dehydrogenase levels. CONCLUSIONS: The development of IL-10-producing Bregs is impaired in patients with advanced MF and a decrease in IL-10-producing Bregs may play an important role in the progression of advanced MF.
BACKGROUND: Historically, B cells have been considered as positive regulators of humoral immune responses. Specific B-cell subsets, however, negatively regulate immune responses and are termed "regulatory B cells" (Bregs). Recently, Bregs have been linked to not only inflammatory and autoimmune diseases, but also malignancies via suppressing anti-tumour immunity. OBJECTIVES: To investigate the involvement of Bregs in advanced mycosis fungoides (MF). MATERIALS & METHODS: The frequency of CD19+CD24hiCD27+ memory B cells and CD19+CD24hiCD38hi transitional B cells (which enrich IL-10-producing Bregs) was examined in peripheral blood from patients with advanced MF (n = 11) and healthy controls (n = 9) by flow cytometry. The frequency of IL-10-producing Bregs was also measured by flow cytometry. The correlation between frequency or number of B-cell subsets and disease severity markers was also analysed. RESULTS: The frequency of CD19+CD24hiCD27+ B cells, CD19+CD24hiCD38hi B cells, and IL-10-producing B cells was decreased in peripheral blood of advanced MF patients. The frequency and number of these B-cell subsets inversely correlated with serum soluble IL-2 receptor and serum lactate dehydrogenase levels. CONCLUSIONS: The development of IL-10-producing Bregs is impaired in patients with advanced MF and a decrease in IL-10-producing Bregs may play an important role in the progression of advanced MF.
Entities:
Keywords:
B cells; IL-10; mycosis fungoides; regulatory B cells (Bregs)
Authors: Pia Rude Nielsen; Jens Ole Eriksen; Mia Dahl Sørensen; Ulrike Wehkamp; Lise M Lindahl; Michael Bzorek; Lars Iversen; Anders Woetman; Niels Ødum; Thomas Litman; Lise Mette Rahbek Gjerdrum Journal: Acta Derm Venereol Date: 2021-03-11 Impact factor: 3.875
Authors: Denis Miyashiro; Bruno de Castro E Souza; Marina Passos Torrealba; Kelly Cristina Gomes Manfrere; Maria Notomi Sato; José Antonio Sanches Journal: Int J Mol Sci Date: 2022-01-15 Impact factor: 5.923