Shey-Ying Chen1, Hao Lee2, Shih-Hua Teng3, Xiao-Mei Wang4, Tai-Fen Lee2, Yu-Chung Huang2,5, Chun-Hsing Liao5, Lee-Jene Teng2,4, Po-Ren Hsueh2,6. 1. Department of Emergency Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan. 2. Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan. 3. Bruker Taiwan Co., Ltd., Taipei, Taiwan. 4. Department of Medical Genetics, National Taiwan University College of Medicine, Taipei, Taiwan. 5. Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City, Taiwan. 6. Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan.
Abstract
AIM: We evaluated a Staphylococcus argenteus-specific diagnostic profile of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for accurate identification of this novel bacterium. MATERIALS & METHODS: Staphylococcus argenteus was identified based on negative crtM gene and presence of specific sequence types. A classification model was generated by cluster analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer results and ClinProTools software for 25 S. argenteus and 25 methicillin-susceptible S. aureus (MSSA). The performance of the classification model was validated against 72 S. argenteus and 72 MSSA isolates. RESULTS: With cluster analysis and classification model, differentiation of 72 S. argenteus from 72 MSSA had 100.0% accuracy by chemical extraction method and 87.5% sensitivity and 100.0% specificity by direct smear method. CONCLUSION: The classification model could accurately differentiate S. argenteus from MSSA.
AIM: We evaluated a Staphylococcus argenteus-specific diagnostic profile of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for accurate identification of this novel bacterium. MATERIALS & METHODS:Staphylococcus argenteus was identified based on negative crtM gene and presence of specific sequence types. A classification model was generated by cluster analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer results and ClinProTools software for 25 S. argenteus and 25 methicillin-susceptible S. aureus (MSSA). The performance of the classification model was validated against 72 S. argenteus and 72 MSSA isolates. RESULTS: With cluster analysis and classification model, differentiation of 72 S. argenteus from 72 MSSA had 100.0% accuracy by chemical extraction method and 87.5% sensitivity and 100.0% specificity by direct smear method. CONCLUSION: The classification model could accurately differentiate S. argenteus from MSSA.
Entities:
Keywords:
Bruker Biotyper MALDI-TOF MS; Staphylococcus argenteus; Staphylococcus aureus; classification model; matrix-assisted laser desorption/ionization-time of flight mass spectrometry
Authors: Eelco F J Meijer; Anne van Renssen; Ianthe Maat; Linda van der Graaf-van Bloois; Birgitta Duim; Els M Broens Journal: Pathogens Date: 2022-01-26