Christine Eymann1,2, Gerhild Wachlin1, Dirk Albrecht1, Stephan Tiede3, Ulrike Krummrei4, Michael Jünger5, Michael Hecker1, Georg Daeschlein5. 1. Institute of Microbiology, Ernst-Moritz-Arndt-University Greifswald, 17489, Greifswald, Germany. 2. Department of Otorhinolaryngology, Head and Neck Surgery, University Medicine Greifswald, 17475, Greifswald, Germany. 3. Institute of Experimental Immunology, EUROIMMUN AG, 17475, Lübeck, Germany. 4. EUROIMMUN Medizinische Labordiagnostika AG, 23560, Lübeck, Germany. 5. Klinik und Poliklinik für Hautkrankheiten, University Medicine Greifswald, 17475, Greifswald, Germany.
Abstract
PURPOSE: Increasing incidence of onychomycosis and tinea pedis in humans of industrialized countries together with deep tissue infections are a therapeutic challenge in clinical mycology. For a better understanding of the pathology and immunology of infection, the authors analyze the exoproteomes of three reference strains of the most common clinical dermatophyte species (Trichophyton rubrum, Trichophyton interdigitale, Arthroderma benhamiae) and of Trichophyton strains isolated from affected patients. EXPERIMENTAL DESIGN: Extracellular proteins of those in vitro grown strains are separated via 2D High Performance Electrophoresis and identified by mass spectrometry to find proteins with provoked host immune reactivity. RESULTS: More than 80 secreted proteins including virulence factors such as peptidases and other hydrolases are identified. By Western blotting with respective patient sera, up to 31 proteins with significant antigen-antibody reactions are detected in comparison with control sera, for example, peptidases as well as several oxidoreductases. One protein, beta-glucosidase F2SZI9 seems to be a commonly processed antigen in all Trichophyton infections. CONCLUSIONS AND CLINICAL RELEVANCE: These first global exoproteome data of three dermatophyte species can be a stepping stone on the way to further study the molecular mechanisms of Trichophyton pathogenicity-associated traits. Possible candidates for potential new diagnostic methods or vaccination have to be validated in further investigations.
PURPOSE: Increasing incidence of onychomycosis and tinea pedis in humans of industrialized countries together with deep tissue infections are a therapeutic challenge in clinical mycology. For a better understanding of the pathology and immunology of infection, the authors analyze the exoproteomes of three reference strains of the most common clinical dermatophyte species (Trichophyton rubrum, Trichophyton interdigitale, Arthroderma benhamiae) and of Trichophyton strains isolated from affected patients. EXPERIMENTAL DESIGN: Extracellular proteins of those in vitro grown strains are separated via 2D High Performance Electrophoresis and identified by mass spectrometry to find proteins with provoked host immune reactivity. RESULTS: More than 80 secreted proteins including virulence factors such as peptidases and other hydrolases are identified. By Western blotting with respective patient sera, up to 31 proteins with significant antigen-antibody reactions are detected in comparison with control sera, for example, peptidases as well as several oxidoreductases. One protein, beta-glucosidase F2SZI9 seems to be a commonly processed antigen in all Trichophytoninfections. CONCLUSIONS AND CLINICAL RELEVANCE: These first global exoproteome data of three dermatophyte species can be a stepping stone on the way to further study the molecular mechanisms of Trichophyton pathogenicity-associated traits. Possible candidates for potential new diagnostic methods or vaccination have to be validated in further investigations.
Authors: Leonardo Martins-Santana; Monise Fazolin Petrucelli; Pablo R Sanches; Nilce M Martinez-Rossi; Antonio Rossi Journal: Front Microbiol Date: 2022-06-17 Impact factor: 6.064
Authors: Verónica L Burstein; Ignacio Beccacece; Lorena Guasconi; Cristian J Mena; Laura Cervi; Laura S Chiapello Journal: Front Immunol Date: 2020-12-02 Impact factor: 7.561