Dong-Hoon Lee1, Do-Wan Lee2, Jae-Im Kwon3, Chul-Woong Woo3, Sang-Tae Kim3, Jin Seong Lee4, Choong Gon Choi4, Kyung Won Kim4, Jeong Kon Kim4, Dong-Cheol Woo5,6. 1. Faculty of Health Sciences and Brain & Mind Centre, The University of Sydney, Sydney, NSW, Australia. 2. Center for Bioimaging of New Drug Development, and MR Core Laboratory, Asan Medical Center, Asan Institute for Life Sciences, Seoul, Republic of Korea. 3. MR Core Laboratory, Asan Medical Center, Asan Institute for Life Sciences, 88, Olympic-ro 43-gil, Songpa-gu, Seoul, 138-736, Republic of Korea. 4. Department of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea. 5. MR Core Laboratory, Asan Medical Center, Asan Institute for Life Sciences, 88, Olympic-ro 43-gil, Songpa-gu, Seoul, 138-736, Republic of Korea. dcwoo@amc.seoul.kr. 6. Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea. dcwoo@amc.seoul.kr.
Abstract
PURPOSE: To evaluate signal changes in the hippocampus of epileptic seizure rat models, based on quantified creatine chemical exchange saturation transfer (CrCEST) signals. PROCEDURES: CEST data and 1H magnetic resonance spectroscopy (1H MRS) data were obtained for the two imaging groups: control (CTRL) and epileptic seizure-induced (ES; via kainic acid [KA] injection) groups. CrCEST signals in the hippocampal regions were quantitatively evaluated; correlations between CrCEST signals and phosphocreatine (PCr) and total creatine (tCr; PCr + Cr) concentrations, derived from the analysis of 1H MRS data, were investigated as a function of time changes (before KA injection, 3 and 5 h after KA injection). RESULTS: Measured CrCEST signals were exhibited significant differences between before and after KA injection in the ES group. At each time point, CrCEST signals showed significant correlations with PCr concentration (all |r| > 0.59; all P < 0.05); no significant correlations were found between CrCEST signals and tCr concentrations (all |r| < 0.22; all P > 0.05). CONCLUSIONS: CrCEST can adequately detect changes in the concentration of Cr as a result of energy metabolism, and may serve as a potentially useful tool for diagnosis and assessment of prognosis in epilepsy.
PURPOSE: To evaluate signal changes in the hippocampus of epileptic seizurerat models, based on quantified creatine chemical exchange saturation transfer (CrCEST) signals. PROCEDURES: CEST data and 1H magnetic resonance spectroscopy (1H MRS) data were obtained for the two imaging groups: control (CTRL) and epileptic seizure-induced (ES; via kainic acid [KA] injection) groups. CrCEST signals in the hippocampal regions were quantitatively evaluated; correlations between CrCEST signals and phosphocreatine (PCr) and total creatine (tCr; PCr + Cr) concentrations, derived from the analysis of 1H MRS data, were investigated as a function of time changes (before KA injection, 3 and 5 h after KA injection). RESULTS: Measured CrCEST signals were exhibited significant differences between before and after KA injection in the ES group. At each time point, CrCEST signals showed significant correlations with PCr concentration (all |r| > 0.59; all P < 0.05); no significant correlations were found between CrCEST signals and tCr concentrations (all |r| < 0.22; all P > 0.05). CONCLUSIONS: CrCEST can adequately detect changes in the concentration of Cr as a result of energy metabolism, and may serve as a potentially useful tool for diagnosis and assessment of prognosis in epilepsy.
Entities:
Keywords:
Chemical exchange saturation transfer; Creatine; Epilepsy; Magnetic resonance spectroscopy; Phosphocreatine
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