Z Zhen1, F Dong, H Shen, Q-G Wang, L Yang, J Hu. 1. Department of Otolaryngology, Head and Neck Surgery, Peking University First Hospital, Beijing, China. bicuculline@gmail.com.
Abstract
OBJECTIVE: To investigate the effect of miR-524 on the proliferation of thyroid cancer and its underlying mechanism. PATIENTS AND METHODS: MiR-524 expression levels in thyroid cancer samples and para-cancer tissues were tested by quantitative Real-time polymerase chain reaction (qRT-PCR). Cells proliferative ability and apoptosis were evaluated through methyl thiazolyl tetrazolium (MTT) and apoptosis assays, respectively. Luciferase reporter assay was used to confirm the regulatory mechanism. RESULTS: MiR-524 expression was reduced in thyroid cancer specimen (p<0.05). Up-regulated miR-524 expression inhibited the proliferative ability and enhanced cell apoptosis of thyroid cancer cells. SPAG9 was a target gene of miR-524, and was reversely regulated by miR-524. CONCLUSIONS: MiR-524 represses thyroid cancer cell proliferation and induces cell apoptosis via targeting SPAG9.
OBJECTIVE: To investigate the effect of miR-524 on the proliferation of thyroid cancer and its underlying mechanism. PATIENTS AND METHODS: MiR-524 expression levels in thyroid cancer samples and para-cancer tissues were tested by quantitative Real-time polymerase chain reaction (qRT-PCR). Cells proliferative ability and apoptosis were evaluated through methyl thiazolyl tetrazolium (MTT) and apoptosis assays, respectively. Luciferase reporter assay was used to confirm the regulatory mechanism. RESULTS: MiR-524 expression was reduced in thyroid cancer specimen (p<0.05). Up-regulated miR-524 expression inhibited the proliferative ability and enhanced cell apoptosis of thyroid cancer cells. SPAG9 was a target gene of miR-524, and was reversely regulated by miR-524. CONCLUSIONS: MiR-524 represses thyroid cancer cell proliferation and induces cell apoptosis via targeting SPAG9.
Authors: Qin Hui Sun; Zong Xiu Yin; Zhi Li; Shu Bo Tian; Hong Chang Wang; Fang Xu Zhang; Le Ping Li; Chun Ning Zheng; Shuai Kong Journal: BMC Cancer Date: 2020-06-05 Impact factor: 4.430