| Literature DB >> 29946362 |
Robert M Hoffman1,2.
Abstract
[This corrects the article DOI: 10.1186/s13039-017-0314-8.].Entities:
Year: 2018 PMID: 29946362 PMCID: PMC6001167 DOI: 10.1186/s13039-018-0385-1
Source DB: PubMed Journal: Mol Cytogenet ISSN: 1755-8166 Impact factor: 2.009
Fig. 1Rates of transmethylation of human tumor cell lines and normal human fibroblast cell strains. All cells were labeled with 100 μM [35S]-methionine-containing medium (25 μCi/ml) for 24 h. Periodateoxidized 3-deazaadenosine was added to a concentration of 10 µM and the accumulation of [35S] AdoHcy was measured at half- hour intervals. Solid lines are human cancer cell lines. Dashed lines are human normal cell strains [38]
Fig. 2Recombinant methioninase (rMETase) traps cancer cells in S/G2 phase. Time-course imaging of HeLa-FUCCI cells treated with rMETase (1.0 unit/ml). Kinetics of rMETase trapping of cells in S/G2. Images were acquired with the FV1000 confocal microscope (Olympus, Tokyo, Japan). In the FUCCI system, the cells in G0/G1, S, or G2/M phases appear red, yellow, or green, respectively [66]
Fig. 3Efficacy of recombinant methioninase (rMETase) on growth of human colon tumors HCT 15 in nude mice. rMETase (5 or 10 units/g every 8 h) was administered by i.p. injection in nude mice with human colon tumor HCT 15, growing s.c. [54]