Literature DB >> 2994632

Preparation of reduced bovine Cu,Zn superoxide dismutase.

P Viglino, M Scarpa, D Cocco, A Rigo.   

Abstract

N.m.r. and e.p.r. were used to measure the oxidation state of copper in Cu,Zn superoxide dismutase treated with reducing agents such as NaBH4, K4Fe(CN)6, Na2S2O4 and H2O2. The activity and the electrophoretic pattern of the treated enzyme were also studied. On the basis of the reducing ability and of the absence of inactivating effects, NaBH4 was the most suitable reducer of those tested. Some characteristics of the reduction of superoxide dismutase by NaBH4 were further investigated. The results obtained indicate that NaBH4 can be used to prepare, in a few minutes, solutions of completely reduced enzyme without any apparent change of the activity and of the structure.

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Year:  1985        PMID： 2994632      PMCID： PMC1145153          DOI： 10.1042/bj2290087

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  Polarographic determination of superoxide dismutase.

Authors:  A Rigo; P Viglino; G Rotilio
Journal:  Anal Biochem       Date:  1975-09       Impact factor: 3.365

2.  The interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide: inactivation of the enzyme.

Authors:  E K Hodgson; I Fridovich
Journal:  Biochemistry       Date:  1975-12-02       Impact factor: 3.162

3.  pH dependece of the nuclear magnetic relaxation rate of solvent water protons in solutions of bovine superoxide dismutase.

Authors:  M Terenzi; A Rigo; C Franconi; L Calabrese; G Rotilio; B Mondovì
Journal:  Biochim Biophys Acta       Date:  1974-06-07

4.  Bovine erythrocyte superoxide dismutase. Subunit structure and sequence location of the intrasubunit disulfide bond.

Authors:  J L Abernethy; H M Steinman; R L Hill
Journal:  J Biol Chem       Date:  1974-11-25       Impact factor: 5.157

5.  Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).

Authors:  J M McCord; I Fridovich
Journal:  J Biol Chem       Date:  1969-11-25       Impact factor: 5.157

6.  An NMR study of the oxidation state of Cu,Zn superoxide dismutase in human red blood cells.

Authors:  M Scarpa; P Viglino; A Rigo
Journal:  Biochem Biophys Res Commun       Date:  1984-08-16       Impact factor: 3.575

7.  Generation of superoxide ion in human red blood cell lysates.

Authors:  M Scarpa; P Viglino; D Contri; A Rigo
Journal:  J Biol Chem       Date:  1984-09-10       Impact factor: 5.157

8.  Reduction and inactivation of superoxide dismutase by hydrogen peroxide.

Authors:  R C Bray; S A Cockle; E M Fielden; P B Roberts; G Rotilio; L Calabrese
Journal:  Biochem J       Date:  1974-04       Impact factor: 3.857

9.  Mechanism of action of superoxide dismutase from pulse radiolysis and electron paramagnetic resonance. Evidence that only half the active sites function in catalysis.

Authors:  E M Fielden; P B Roberts; R C Bray; D J Lowe; G N Mautner; G Rotilio; L Calabrese
Journal:  Biochem J       Date:  1974-04       Impact factor: 3.857

10.  The binding of copper ions to copper-free bovine superoxide dismutase. Copper distribution in protein samples recombined with less than stoicheiometric copper ion/protein ratios.

Authors:  A Rigo; P Viglino; L Calabrese; D Cocco; G Rotilio
Journal:  Biochem J       Date:  1977-01-01       Impact factor: 3.857
  10 in total
  2 in total

1.  Oxidation of reduced Cu,Zn superoxide dismutase by molecular oxygen. A kinetic study.

Authors:  P Viglino; M Scarpa; F Coin; G Rotilio; A Rigo
Journal:  Biochem J       Date:  1986-07-01       Impact factor: 3.857

2.  Reversible conversion of nitroxyl anion to nitric oxide by superoxide dismutase.

Authors:  M E Murphy; H Sies
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-01       Impact factor: 11.205
  2 in total

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