Fábio P Sellera1, Miriam R Fernandes2, Quézia Moura3, Ralf B Lopes3, Tiago A Souza4, Louise Cerdeira2, Nilton Lincopan5. 1. Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil. 2. Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil. 3. Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. 4. Genome Investigation and Analysis Laboratory (GENIAL), Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. 5. Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: lincopan@usp.br.
Abstract
OBJECTIVES: Marine bivalves can act as bioindicators of marine environment pollution by multidrug-resistant (MDR) enteric bacteria of medical interest. The aim of this study was to report the draft genome sequence of a plasmid-encoded AmpC (pAmpC) (CMY-2)-carrying Escherichia coli isolate recovered from a marine bivalve sample in the coastal shore of Southeast Brazil. METHODS: The whole genome was sequenced on an Illumina NextSeq platform and was assembled using Velvet v.1.2.10. Data analysis was carried out using tools available from the Center of Genomic Epidemiology and Geneious R10 software. RESULTS: The genome size was calculated at 5198055bp, comprising a total of 5316 protein-coding sequences. The strain was assigned to ST457 and presented the blaCMY-2 pAmpC gene. In addition, the strain was clustered into the pathogenic phylogenetic group D. CONCLUSION: The release of this draft genome sequence can provide valuable information to better understand the dissemination of MDR enteric bacteria in marine environments.
OBJECTIVES: Marine bivalves can act as bioindicators of marine environment pollution by multidrug-resistant (MDR) enteric bacteria of medical interest. The aim of this study was to report the draft genome sequence of a plasmid-encoded AmpC (pAmpC) (CMY-2)-carrying Escherichia coli isolate recovered from a marine bivalve sample in the coastal shore of Southeast Brazil. METHODS: The whole genome was sequenced on an Illumina NextSeq platform and was assembled using Velvet v.1.2.10. Data analysis was carried out using tools available from the Center of Genomic Epidemiology and Geneious R10 software. RESULTS: The genome size was calculated at 5198055bp, comprising a total of 5316 protein-coding sequences. The strain was assigned to ST457 and presented the blaCMY-2 pAmpC gene. In addition, the strain was clustered into the pathogenic phylogenetic group D. CONCLUSION: The release of this draft genome sequence can provide valuable information to better understand the dissemination of MDR enteric bacteria in marine environments.
Authors: Fábio P Sellera; Brenda Cardoso; Danny Fuentes-Castillo; Fernanda Esposito; Elder Sano; Herrison Fontana; Bruna Fuga; Daphne W Goldberg; Lourdes A V Seabra; Marzia Antonelli; Sandro Sandri; Cristiane K M Kolesnikovas; Nilton Lincopan Journal: Front Microbiol Date: 2022-06-10 Impact factor: 6.064
Authors: Steven P Djordjevic; Monika Dolejska; Kristina Nesporova; Ethan R Wyrsch; Adam Valcek; Ibrahim Bitar; Khin Chaw; Patrick Harris; Jaroslav Hrabak; Ivan Literak Journal: Antimicrob Agents Chemother Date: 2020-12-16 Impact factor: 5.191
Authors: Vanessa Bueris; Fábio P Sellera; Bruna Fuga; Elder Sano; Marcelo P N Carvalho; Samuel C F Couto; Quézia Moura; Nilton Lincopan Journal: Sci Rep Date: 2022-04-05 Impact factor: 4.379
Authors: Ana Carolina Ewbank; Danny Fuentes-Castillo; Carlos Sacristán; Fernanda Esposito; Bruna Fuga; Brenda Cardoso; Silvia Neri Godoy; Roberta Ramblas Zamana; Marco Aurélio Gattamorta; José Luiz Catão-Dias; Nilton Lincopan Journal: Front Microbiol Date: 2022-08-11 Impact factor: 6.064