| Literature DB >> 2994278 |
H J Chu, Y C Zee, A A Ardans, K Dai.
Abstract
A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was established for the detection of antibodies to bovine viral diarrhea virus (BVDV) in bovine sera. Polyethylene-glycol concentrated, equilibrium density gradient purified BVDV was used as test antigen at an optimal amount of 1 microgram/well, whereas the optimal concentration of conjugate was at 1/2000 dilution. The standardized test encountered no non-specific reaction with test sera at a starting dilution of 1/10. A total of 50 bovine serum samples was assayed for the presence of antibodies against BVDV by ELISA and serum neutralization test (SNT). A positive correlation between the 2 tests was found. However, ELISA could be as much as 500-fold more sensitive than SNT in detecting low levels of BVDV antibodies.Entities:
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Year: 1985 PMID： 2994278 DOI： 10.1016/0378-1135(85)90003-3
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293