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Literature DB >> 2994034

The large scale purification of ubiquitin from human erythrocytes.

Abstract

A simple, reproducible method for the large-scale purification of active ubiquitin from human erythrocytes is described. Erythrocytes contain 100 micrograms free ubiquitin per cc of packed cells, of which 44% can be recovered in homogeneous form by a combination of heat treatment, ammonium sulfate fractionation, and ion exchange chromatography.

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Year: 1985 PMID： 2994034 DOI： 10.1080/00327488508062433

Source DB: PubMed Journal: Prep Biochem ISSN： 0032-7484

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Cited

12 in total

1. A ubiquitin carrier protein from wheat germ is structurally and functionally similar to the yeast DNA repair enzyme encoded by RAD6.

Authors: M L Sullivan; R D Vierstra
Journal: Proc Natl Acad Sci U S A Date: 1989-12 Impact factor: 11.205

2. Alzheimer's disease brain-derived ubiquitin has amyloid-enhancing factor activity: behavior of ubiquitin during accelerated amyloidogenesis.

Authors: K Alizadeh-Khiavi; J Normand; S Chronopoulos; Z Ali-Khan
Journal: Acta Neuropathol Date: 1991 Impact factor: 17.088

3. Cloning and characterization of a 20-kDa ubiquitin carrier protein from wheat that catalyzes multiubiquitin chain formation in vitro.

Authors: S Van Nocker; R D Vierstra
Journal: Proc Natl Acad Sci U S A Date: 1991-11-15 Impact factor: 11.205

The large scale purification of ubiquitin from human erythrocytes.

1. A ubiquitin carrier protein from wheat germ is structurally and functionally similar to the yeast DNA repair enzyme encoded by RAD6.

2. Alzheimer's disease brain-derived ubiquitin has amyloid-enhancing factor activity: behavior of ubiquitin during accelerated amyloidogenesis.

3. Cloning and characterization of a 20-kDa ubiquitin carrier protein from wheat that catalyzes multiubiquitin chain formation in vitro.

4. A specific endpoint assay for ubiquitin.

5. Ubiquitin has intrinsic proteolytic activity: implications for cellular regulation.

6. Characterization of a polyubiquitin gene from Arabidopsis thaliana.

7. Cellular content of ubiquitin and formation of ubiquitin conjugates during chicken spermatogenesis.

8. Demonstration of ATP-Dependent, Ubiquitin-Conjugating Activities in Higher Plants.

9. Increased extracellular ubiquitin in surgical wound fluid provides a chemotactic signal for myeloid dendritic cells.

10. Auto-ubiquitination of ubiquitin-activating enzymes from chicken breast muscle.