Sascha D Braun1,2, Bushra Jamil3, Muhammad A Syed4, Shahid A Abbasi5, Daniel Weiß1,2, Peter Slickers1,2, Stefan Monecke1,2,6, Ines Engelmann1,2, Ralf Ehricht1,2. 1. Research & Development, Abbott (Alere Technologies GmbH), Jena, Germany. 2. InfectoGnostics Research Campus, Jena, Germany. 3. Department of Biogenetics, National University of Medical Sciences, Rawalpindi, Pakistan. 4. Department of Microbiology, University of Haripur, Haripur, Pakistan. 5. Department of Pathology, Al-Sayed Hospital (Pvt) Ltd, 1-Hill Park, Opp. Ayub Park, Jhelum Road, Rawalpindi, Pakistan. 6. Technische Universität Dresden, Medizinische Fakultät "Carl Gustav Carus", Dresden, Germany.
Abstract
AIM: A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan. METHODS: The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical samples identified 425 Gram-negative bacteria out of which 82 appeared carbapenem resistant. These isolates were analyzed using VITEK-2 for phenotyping and the described AMR assay for genotyping. RESULTS: The most prevalent carbapenemase gene was blaNDM and in 12 isolates we detected two carbapenemase genes (e.g., blaNDM/blaOXA-48). CONCLUSION: Our prevalence data from Pakistan show that - as in other parts of the world - carbapenemase-producing organisms with different underlying resistance mechanisms are emerging, and this warrants intensified and constant surveillance.
AIM: A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan. METHODS: The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical samples identified 425 Gram-negative bacteria out of which 82 appeared carbapenem resistant. These isolates were analyzed using VITEK-2 for phenotyping and the described AMR assay for genotyping. RESULTS: The most prevalent carbapenemase gene was blaNDM and in 12 isolates we detected two carbapenemase genes (e.g., blaNDM/blaOXA-48). CONCLUSION: Our prevalence data from Pakistan show that - as in other parts of the world - carbapenemase-producing organisms with different underlying resistance mechanisms are emerging, and this warrants intensified and constant surveillance.
Authors: Samyyia Abrar; Noor Ul Ain; Huma Liaqat; Shahida Hussain; Farhan Rasheed; Saba Riaz Journal: Antimicrob Resist Infect Control Date: 2019-05-22 Impact factor: 4.887
Authors: Olivia M Grünzweil; Lauren Palmer; Adriana Cabal; Michael P Szostak; Werner Ruppitsch; Christian Kornschober; Maciej Korus; Dusan Misic; Tanja Bernreiter-Hofer; Anna D J Korath; Andrea T Feßler; Franz Allerberger; Stefan Schwarz; Joachim Spergser; Elke Müller; Sascha D Braun; Stefan Monecke; Ralf Ehricht; Chris Walzer; Hrvoje Smodlaka; Igor Loncaric Journal: Int J Mol Sci Date: 2021-05-31 Impact factor: 5.923