Literature DB >> 2993815

Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other gram negative bacteria.

G J Boulnois, J M Varley, G S Sharpe, F C Franklin.   

Abstract

The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a similar plasmid pLG223 carrying Tn10 may be of general utility as vectors for transposon-mediated mutagenesis in a variety of Gram negative bacteria.

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Year:  1985        PMID: 2993815     DOI: 10.1007/bf00383313

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN: 0026-8925


  16 in total

1.  PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT.

Authors:  H SAITO; K I MIURA
Journal:  Biochim Biophys Acta       Date:  1963-08-20

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance.

Authors:  R A Jorgensen; S J Rothstein; W S Reznikoff
Journal:  Mol Gen Genet       Date:  1979

4.  A novel priming system for conjugal synthesis of an IncI alpha plasmid in recipients.

Authors:  G J Boulnois; B M Wilkins
Journal:  Mol Gen Genet       Date:  1979-10-01

5.  Identification of gene products programmed by restriction endonuclease DNA fragments using an E. coli in vitro system.

Authors:  J M Pratt; G J Boulnois; V Darby; E Orr; E Wahle; I B Holland
Journal:  Nucleic Acids Res       Date:  1981-09-25       Impact factor: 16.971

6.  Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway.

Authors:  F C Franklin; M Bagdasarian; M M Bagdasarian; K N Timmis
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

7.  Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid.

Authors:  P A Williams; K Murray
Journal:  J Bacteriol       Date:  1974-10       Impact factor: 3.490

8.  Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.

Authors:  M Bagdasarian; R Lurz; B Rückert; F C Franklin; M M Bagdasarian; J Frey; K N Timmis
Journal:  Gene       Date:  1981-12       Impact factor: 3.688

9.  Involvement of kil and kor genes in the phenotype of a host-range mutant of RP4.

Authors:  P T Barth; K Ellis; D H Bechhofer; D H Figurski
Journal:  Mol Gen Genet       Date:  1984

10.  A plasmid DNA primase active in discontinuous bacterial DNA replication.

Authors:  B M Wilkins; G J Boulnois; E Lanka
Journal:  Nature       Date:  1981-03-19       Impact factor: 49.962

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  11 in total

1.  Cloning and analysis of s-triazine catabolic genes from Pseudomonas sp. strain NRRLB-12227.

Authors:  R W Eaton; J S Karns
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490

2.  Isolation of high frequency of recombination donors from Tn5 chromosomal mutants of Pseudomonas putida PPN and recalibration of the genetic map.

Authors:  A D Strom; R Hirst; J Petering; A Morgan
Journal:  Genetics       Date:  1990-11       Impact factor: 4.562

3.  The sigma factor AlgU (AlgT) controls exopolysaccharide production and tolerance towards desiccation and osmotic stress in the biocontrol agent Pseudomonas fluorescens CHA0.

Authors:  U Schnider-Keel; K B Lejbølle; E Baehler; D Haas; C Keel
Journal:  Appl Environ Microbiol       Date:  2001-12       Impact factor: 4.792

4.  Autoinduction of 2,4-diacetylphloroglucinol biosynthesis in the biocontrol agent Pseudomonas fluorescens CHA0 and repression by the bacterial metabolites salicylate and pyoluteorin.

Authors:  U Schnider-Keel; A Seematter; M Maurhofer; C Blumer; B Duffy; C Gigot-Bonnefoy; C Reimmann; R Notz; G Défago; D Haas; C Keel
Journal:  J Bacteriol       Date:  2000-03       Impact factor: 3.490

5.  Characterization of a plasmid-specified pathway for catabolism of isopropylbenzene in Pseudomonas putida RE204.

Authors:  R W Eaton; K N Timmis
Journal:  J Bacteriol       Date:  1986-10       Impact factor: 3.490

6.  Thiamine-auxotrophic mutants of Pseudomonas fluorescens CHA0 are defective in cell-cell signaling and biocontrol factor expression.

Authors:  Christophe Dubuis; Joëlle Rolli; Matthias Lutz; Geneviève Défago; Dieter Haas
Journal:  Appl Environ Microbiol       Date:  2006-04       Impact factor: 4.792

7.  Survival of Pseudomonas putida UWC1 containing cloned catabolic genes in a model activated-sludge unit.

Authors:  N C McClure; A J Weightman; J C Fry
Journal:  Appl Environ Microbiol       Date:  1989-10       Impact factor: 4.792

8.  Conjugative Transfer of Chromosomal Genes between Fluorescent Pseudomonads in the Rhizosphere of Wheat.

Authors:  J Troxler; P Azelvandre; M Zala; G Defago; D Haas
Journal:  Appl Environ Microbiol       Date:  1997-01       Impact factor: 4.792

9.  Enumeration of Tn5 mutant bacteria in soil by using a most- probable-number-DNA hybridization procedure and antibiotic resistance.

Authors:  J K Fredrickson; D F Bezdicek; F J Brockman; S W Li
Journal:  Appl Environ Microbiol       Date:  1988-02       Impact factor: 4.792

10.  Localization and functional analysis of structural and regulatory dehalogenase genes carried on DEH from Pseudomonas putida PP3.

Authors:  A W Thomas; A W Topping; J H Slater; A J Weightman
Journal:  J Bacteriol       Date:  1992-03       Impact factor: 3.490

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