| Literature DB >> 2993815 |
G J Boulnois, J M Varley, G S Sharpe, F C Franklin.
Abstract
The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a similar plasmid pLG223 carrying Tn10 may be of general utility as vectors for transposon-mediated mutagenesis in a variety of Gram negative bacteria.Entities:
Mesh:
Substances:
Year: 1985 PMID: 2993815 DOI: 10.1007/bf00383313
Source DB: PubMed Journal: Mol Gen Genet ISSN: 0026-8925