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Literature DB >> 2993800

Rec-dependent and Rec-independent recombination of plasmid-borne duplications in Escherichia coli K12.

Abstract

Plasmidic recombination in E. coli K12 has been previously demonstrated to be dependent on the host rec genotype. The construction of plasmids that carry a duplication within an antibiotic-resistance gene is described. Recombination between the direct repeats recreates an active antibiotic-resistance gene, allowing quantitative analysis of recombination frequencies in a closely related set of E. coli K12 strains carrying various rec mutations. Using this system, intraplasmidic recombination of a duplication within the pBR322 tetracycline-resistance gene is shown to be rec-dependent while recombination of a similar duplication within the kanamycin-resistance gene of Tn903 is shown to be independent of recA, recB, recC, recE, recF and sbcB.

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Year: 1985 PMID： 2993800 DOI： 10.1007/bf00330768

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

30 in total

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Journal: Gene Date: 1978-04 Impact factor: 3.688

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Journal: Mol Gen Genet Date: 1982

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Journal: Mol Gen Genet Date: 1980

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Journal: Nucleic Acids Res Date: 1979-07-25 Impact factor: 16.971

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Authors: M Clerget
Journal: J Mol Biol Date: 1984-09-05 Impact factor: 5.469

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Journal: Cell Date: 1977-03 Impact factor: 41.582

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Authors: D Hanahan
Journal: J Mol Biol Date: 1983-06-05 Impact factor: 5.469

13 in total

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Authors: M Bzymek; S T Lovett
Journal: Proc Natl Acad Sci U S A Date: 2001-07-17 Impact factor: 11.205

2. The rational design of a 'type 88' genetically stable peptide display vector in the filamentous bacteriophage fd.

Authors: D Enshell-Seijffers; L Smelyanski; J M Gershoni
Journal: Nucleic Acids Res Date: 2001-05-15 Impact factor: 16.971

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Authors: C T Lin; Y L Lyu; L F Liu
Journal: Nucleic Acids Res Date: 1997-08-01 Impact factor: 16.971

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Authors: G L Dianov; A V Kuzminov; A V Mazin; R I Salganik
Journal: Mol Gen Genet Date: 1991-08

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Authors: G Levinson; G A Gutman
Journal: Nucleic Acids Res Date: 1987-07-10 Impact factor: 16.971