Ahmad Mouwakeh1, Ágnes Telbisz2, Gabriella Spengler3, Csilla Mohácsi-Farkas1, Gabriella Kiskó4. 1. Department of Microbiology and Biotechnology, Szent Istvan University, Budapest, Hungary. 2. Institute of Enzymology, Hungarian Academy of Science, Budapest, Hungary. 3. Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary. 4. Department of Microbiology and Biotechnology, Szent Istvan University, Budapest, Hungary gabriella.kisko@etk.szie.hu.
Abstract
BACKGROUND/AIM: N. sativa essential oil (EO) and its compounds (thymoquinone, carvacrol and p-cymene) have a broad antimicrobial spectrum. The aim of this study was to investigate the antimicrobial and resistance modifying activity of N. sativa EO, thymoquinone, carvacrol and p-cymene against Listeria monocytogenes. MATERIALS AND METHODS: N. sativa EO, thymoquinone, carvacrol and p-cymene was assessed for its antimicrobial activity, modulation of antimicrobial resistance, inhibition of antimicrobial efflux and membrane integrity by broth microdilution, ethidium bromide accumulation and LIVE/DEAD BacLight™ assays. RESULTS: L. monocytogenes showed substantial susceptibility toward N. sativa EO, thymoquinone, and carvacrol. A significant reduction in MIC's of EtBr and ciprofloxacin was noticed when tested in combination with N. sativa EO, thymoquinone, carvacrol and reserpine. In the presence of each compound the membrane integrity was disintegrated, and the EtBr accumulation increased which was comparable to positive control reserpine. CONCLUSION: N. sativa EO might have a potential for controlling the antibiotic resistance in Listeria. Copyright
BACKGROUND/AIM: N. sativa essential oil (EO) and its compounds (thymoquinone, carvacrol and p-cymene) have a broad antimicrobial spectrum. The aim of this study was to investigate the antimicrobial and resistance modifying activity of N. sativaEO, thymoquinone, carvacrol and p-cymene against Listeria monocytogenes. MATERIALS AND METHODS:N. sativaEO, thymoquinone, carvacrol and p-cymene was assessed for its antimicrobial activity, modulation of antimicrobial resistance, inhibition of antimicrobial efflux and membrane integrity by broth microdilution, ethidium bromide accumulation and LIVE/DEAD BacLight™ assays. RESULTS:L. monocytogenes showed substantial susceptibility toward N. sativaEO, thymoquinone, and carvacrol. A significant reduction in MIC's of EtBr and ciprofloxacin was noticed when tested in combination with N. sativaEO, thymoquinone, carvacrol and reserpine. In the presence of each compound the membrane integrity was disintegrated, and the EtBr accumulation increased which was comparable to positive control reserpine. CONCLUSION:N. sativaEO might have a potential for controlling the antibiotic resistance in Listeria. Copyright
Authors: Mohamed F R Hassanien; Adel M A Assiri; Ahmed M Alzohairy; Hesham Farouk Oraby Journal: J Food Sci Technol Date: 2015-03-27 Impact factor: 2.701
Authors: Winfried V Kern; Petra Steinke; Anja Schumacher; Sabine Schuster; Heike von Baum; Jürgen A Bohnert Journal: J Antimicrob Chemother Date: 2005-12-14 Impact factor: 5.790
Authors: Ayed A Dera; Irfan Ahmad; Prasanna Rajagopalan; Mesfer Al Shahrani; Ahmed Saif; Mohammad Y Alshahrani; Yasser Alraey; Ahmad M Alamri; Sultan Alasmari; Mohammed Makkawi; Ali G Alkhathami; Gaffar Zaman; Abdulrahim Hakami; Razan Alhefzi; Mohammad A Alfhili Journal: Saudi Med J Date: 2021-02 Impact factor: 1.484
Authors: Mohammed S Algahtani; Mohammad Zaki Ahmad; Ibrahim Ahmed Shaikh; Basel A Abdel-Wahab; Ihab Hamed Nourein; Javed Ahmad Journal: Molecules Date: 2021-06-24 Impact factor: 4.411