Martin Freesmeyer1, Christian Kuehnel2, Thomas Opfermann2, Tobias Niksch2, Steffen Wiegand2, Ronny Stolz3, Ralph Huonker4, Otto W Witte4,5, Thomas Winkens2. 1. Clinic of Nuclear Medicine, Jena University Hospital, Jena, Germany martin.freesmeyer@med.uni-jena.de. 2. Clinic of Nuclear Medicine, Jena University Hospital, Jena, Germany. 3. Leibniz Institute of Photonic Technology, Jena, Germany. 4. Biomagnetic Center, Hans Berger Department of Neurology, Jena University Hospital, Jena, Germany; and. 5. Hans Berger Department of Neurology, Jena University Hospital, Jena, Germany.
Abstract
In ovo studies are a valuable option in preclinical research, but imaging studies are severely limited by the costs of dedicated equipment needed for small-sized eggs. We sought to verify the feasibility of using larger, ostrich, eggs (Struthio camelus) for imaging on the PET/CT scanners used for routine clinical investigations. Methods: Ostrich eggs were incubated until shortly before hatching, prepared for intravitelline venous injection of contrast medium or radiotracer, and imaged using native CT, contrast-enhanced CT, and PET/CT. Any technical adaptations that were needed to improve the outcome were noted. Results: Of the 34 eggs initially incubated, 12 became fully available for imaging of embryonal development. In ovo imaging with conventional PET/CT not only was feasible but also provided images of good quality, including on dynamic PET imaging. Conclusion: In ovo imaging with ostrich eggs and routine clinical scanners may allow broader application of this field of preclinical research, obviating costly dedicated equipment and reducing the number of animals needed for classic animal research. Further experiments are warranted to refine this novel approach, especially to reduce motion artifacts and improve monitoring of viability.
In ovo studies are a valuable option in preclinical research, but imaging studies are severely limited by the costs of dedicated equipment needed for small-sized eggs. We sought to verify the feasibility of using larger, ostrich, eggs (Struthio camelus) for imaging on the PET/CT scanners used for routine clinical investigations. Methods:Ostrich eggs were incubated until shortly before hatching, prepared for intravitelline venous injection of contrast medium or radiotracer, and imaged using native CT, contrast-enhanced CT, and PET/CT. Any technical adaptations that were needed to improve the outcome were noted. Results: Of the 34 eggs initially incubated, 12 became fully available for imaging of embryonal development. In ovo imaging with conventional PET/CT not only was feasible but also provided images of good quality, including on dynamic PET imaging. Conclusion: In ovo imaging with ostrich eggs and routine clinical scanners may allow broader application of this field of preclinical research, obviating costly dedicated equipment and reducing the number of animals needed for classic animal research. Further experiments are warranted to refine this novel approach, especially to reduce motion artifacts and improve monitoring of viability.
Authors: Martin Freesmeyer; Hanna Hermeyer; Christian Kuehnel; Olga Perkas; Julia Greiser; Otto W Witte; Thomas Winkens Journal: Exp Biol Med (Maywood) Date: 2022-04-25
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