| Literature DB >> 29934148 |
David Romera1, Eva M Mateo1, Rufino Mateo-Castro2, José V Gómez1, José V Gimeno-Adelantado2, Misericordia Jiménez3.
Abstract
In this report, a UPLC-ESI-MS/MS method for the simultaneous determination of aflatoxins, ochratoxin A, zearalenone, deoxynivalenol, fumonisins, T-2 and HT-2 toxins, fusarenone X, diacetoxyscirpenol, and 3- and 15-acetyldeoxynivalenol in feedstuffs was developed. A quadrupole-time-of-flight mass spectrometer detector (QTOF-MS) operating in full scan mode was combined with the UPLC-ESI-MS/MS system to confirm the identity of detected mycotoxins and to identify other possible microbial metabolites occurring in samples. Sixty-two feed samples from the Spanish market were analyzed. Extraction of metabolites was carried out with acetonitrile-water-formic acid (80:19:1, v/v/v). Method detection and quantification limits and performance criteria set by Commission Regulation (EC) No 401/2006 were fulfilled. Relatively high levels of the main regulated mycotoxins and presence of non-regulated mycotoxins in feed samples were found. This is the first study in which mycotoxins and other microbial metabolites occurring in feed are studied using a UPLC-QTOF-MS system being therefore a reference report.Entities:
Keywords: Feedstuffs; Liquid chromatography; Mass spectrometry; Mycotoxins; UPLC–MS/MS; UPLC−QTOF−MS
Mesh：
Substances：
Year: 2017 PMID： 29934148 DOI： 10.1016/j.foodchem.2017.11.040
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514