Literature DB >> 29930

Organic solvents and temperature effects on desorption from immunoadsorbents. DNP-BSA anti-DNP as a model.

K Andersson, Y Benyamin, P Douzou, C Balny.   

Abstract

Conditions for desorbing a DNP carrier from its immunoadsorbent were studied in various hydro-organic media at different pHs and temperatures, including the sub-zero range. It appeared that DNP--anti-DNP interaction is the result of a balance between various bonds and the best result (95% yield) was obtained in hydro-organic solvent at high pH and +30 degrees C.

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Year:  1978        PMID： 29930     DOI： 10.1016/0022-1759(78)90105-9

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  6 in total

1.  Structural variations in actins. A study of the immunological reactivity of the N-terminal region.

Authors:  C Roustan; Y Benyamin; M Boyer; J C Cavadore
Journal:  Biochem J       Date:  1986-01-01       Impact factor: 3.857

2.  Antigenic probes locate a serum-gelsolin-interaction site on the C-terminal part of actin.

Authors:  M Boyer; J Feinberg; H K Hue; J P Capony; Y Benyamin; C Roustan
Journal:  Biochem J       Date:  1987-12-01       Impact factor: 3.857

3.  Gibberellic acid and abscisic acid modulate protein synthesis and mRNA levels in barley aleurone layers.

Authors:  T J Higgins; J V Jacobsen; J A Zwar
Journal:  Plant Mol Biol       Date:  1982-09       Impact factor: 4.076

4.  Immunological comparison between albumins of three species of mice (genus Mus).

Authors:  C Montgelard; Y Benyamin; C Roustan
Journal:  Experientia       Date:  1990-03-15

5.  Definition of a Ca2(+)-sensitive interface in the plasma gelsolin-actin complex.

Authors:  A Houmeida; V Hanin; J Feinberg; Y Benyamin; C Roustan
Journal:  Biochem J       Date:  1991-03-15       Impact factor: 3.857

6.  Actin antibodies. Preparation and characterization of antibodies specific for smooth-muscle actin isoforms.

Authors:  J C Cavadore; F Martin; B Calas; J Mery; P Berta; Y Benyamin; C Roustan
Journal:  Biochem J       Date:  1987-02-15       Impact factor: 3.857
  6 in total

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