Sandra López-Domènech1, Celia Bañuls1,2, Noelia Díaz-Morales1, Irene Escribano-López1, Carlos Morillas1, Silvia Veses1, Samuel Orden3, Ángeles Álvarez3,4, Víctor M Víctor1,2,3,5, Antonio Hernández-Mijares1,2,6, Milagros Rocha1,2,3. 1. Service of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Valencia, Spain. 2. Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain. 3. CIBER CB06/04/0071 Research Group, CIBER Hepatic and Digestive Diseases, University of Valencia, Valencia, Spain. 4. Facultad de Ciencias de la Salud, Universidad Jaume I, Castellón de la Plana, Spain. 5. Department of Physiology, University of Valencia, Valencia, Spain. 6. Department of Medicine, University of Valencia, Valencia, Spain.
Abstract
BACKGROUND: To evaluate the relationship between leukocyte-endothelial cell interactions and oxidative stress parameters in non-diabetic patients with different grades of obesity. MATERIAL AND METHODS: For this cross-sectional study, 225 subjects were recruited from January 1, 2014 to December 31, 2016 and divided into groups according to BMI (<30 kg/m2 , 30-40 kg/m2 and >40 kg/m²). We determined clinical parameters, systemic inflammatory markers, soluble cellular adhesion molecules, leukocyte-endothelium cell interactions-rolling flux, velocity and adhesion-, oxidative stress parameters-total ROS, total superoxide, glutathione-and mitochondrial membrane potential in leukocytes. RESULTS: We verified that HOMA-IR and hsCRP increased progressively as obesity developed, whereas A1c, IL6 and TNFα were augmented in the BMI > 40 kg/m² group. The cellular adhesion molecule sP-selectin was increased in patients with obesity, while sICAM, total ROS, total superoxide and mitochondrial membrane potential were selectively higher in the BMI > 40 kg/m² group. Obesity induced a progressive decrease in rolling velocity and an enhancement of rolling flux and leukocyte adhesion. CONCLUSION: Our findings reveal that endothelial dysfunction markers are altered in human obesity and are associated with proinflammatory cytokines and increased oxidative stress parameters.
BACKGROUND: To evaluate the relationship between leukocyte-endothelial cell interactions and oxidative stress parameters in non-diabeticpatients with different grades of obesity. MATERIAL AND METHODS: For this cross-sectional study, 225 subjects were recruited from January 1, 2014 to December 31, 2016 and divided into groups according to BMI (<30 kg/m2 , 30-40 kg/m2 and >40 kg/m²). We determined clinical parameters, systemic inflammatory markers, soluble cellular adhesion molecules, leukocyte-endothelium cell interactions-rolling flux, velocity and adhesion-, oxidative stress parameters-total ROS, total superoxide, glutathione-and mitochondrial membrane potential in leukocytes. RESULTS: We verified that HOMA-IR and hsCRP increased progressively as obesity developed, whereas A1c, IL6 and TNFα were augmented in the BMI > 40 kg/m² group. The cellular adhesion molecule sP-selectin was increased in patients with obesity, while sICAM, total ROS, total superoxide and mitochondrial membrane potential were selectively higher in the BMI > 40 kg/m² group. Obesity induced a progressive decrease in rolling velocity and an enhancement of rolling flux and leukocyte adhesion. CONCLUSION: Our findings reveal that endothelial dysfunction markers are altered in humanobesity and are associated with proinflammatory cytokines and increased oxidative stress parameters.
Authors: Giovanni Pagano; Federico V Pallardó; Beatriz Porto; Maria Rosa Fittipaldi; Alex Lyakhovich; Marco Trifuoggi Journal: Antioxidants (Basel) Date: 2020-01-18