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Literature DB >> 2992372

Evidence for IFN-beta heterogeneity in a substrain of Namalwa cells.

Abstract

A substrain of Namalwa cells, denoted substrain B, was grown in fermentors up to the 100-L scale, and was induced with Sendai virus to produce interferon (IFN). The titer of the crude IFN varied extensively between different batches; part of the variation was caused by a differential expression of IFN-alpha and IFN-beta. More than 80% of the IFN activity was IFN-beta by several criteria. A two-step purification procedure was developed and the resulting preparation had a specific activity of approximately 10(6) U/mg protein. The IFN-beta type was found to be heterogeneous, and could be separated into several components, which probably represented post-translational modifications of one molecule.

Entities: Gene Species

Mesh：

Substances：
Interferon Type I

Year: 1985 PMID： 2992372 DOI： 10.1007/bf02798543

Source DB: PubMed Journal: Appl Biochem Biotechnol ISSN： 0273-2289 Impact factor: 2.926

17 in total

1. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus.

Authors: G Klein; L Dombos; B Gothoskar
Journal: Int J Cancer Date: 1972-07-15 Impact factor: 7.396

2. Production of high-titered interferon in cultures of human diploid cells.

Authors: E A Havell; J Vilcek
Journal: Antimicrob Agents Chemother Date: 1972-12 Impact factor: 5.191

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

4. The characteristics required for a Sendai virus preparation to induce high levels of interferon in human lymphoblastoid cells.

Authors: M D Johnston
Journal: J Gen Virol Date: 1981-09 Impact factor: 3.891

Evidence for IFN-beta heterogeneity in a substrain of Namalwa cells.

1. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus.

2. Production of high-titered interferon in cultures of human diploid cells.

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

4. The characteristics required for a Sendai virus preparation to induce high levels of interferon in human lymphoblastoid cells.

5. The identity of interferon-beta 1 mRNA transcripts in human fibroblasts and Namalva cells.

6. Structure and properties of human interferon-alpha from Namalwa lymphoblastoid cells.

7. Use of polyethylene glycol-treated calf serum for cell cultures in virus and interferon studies.

8. Biological properties of human interferon fractions obtained by blue sepharose chromatography.

9. Differences in sialic acid content of human interferons.

10. Expression of interferon-alpha and interferon-beta genes in human lymphoblastoid (Namalwa) cells.

1. Repression of beta interferon gene expression in virus-infected cells is correlated with a poly(A) tail elongation.