Ali Güleç1, Yılmaz Türk1, Bahattin Kerem Aydin2, Ömer Faruk Erkoçak1, Selim Safalı1, Ceyhan Ugurluoglu3. 1. Department of Orthopaedics and Traumatology, Faculty of Medicine, Selcuk University, Alaeddin Keykubat Campus, 42130, Konya, Turkey. 2. Department of Orthopaedics and Traumatology, Faculty of Medicine, Selcuk University, Alaeddin Keykubat Campus, 42130, Konya, Turkey. bkaydin@yahoo.com. 3. Department of Pathology, Faculty of Medicine, Selcuk University, Alaeddin Keykubat Campus, 42130, Konya, Turkey.
Abstract
PURPOSE: This in vivo study aims to investigate the effects of curcumin which is recently developed for tendon healing using a rat Achilles tendon injury model. MATERIALS AND METHODS: Eighteen male Wistar albino rats weighing 300-400 g were used in this study. Under anesthesia, Achilles tendon injuries were created and repaired surgically. Nine rats of the study group received curcumin (suspended in saline at a dose of 200 mg/kg orally) and eight rats of the control group received only saline solution by oral gavage for a period of 28 days. Animals were euthanized on the 28th post-operative day, and all the Achilles tendons were removed and transferred immediately for biomechanic and histological analysis. RESULTS: Macroscopically, all the tendons were fully healed. Total mean Bonar score was higher in the control group. When the parameters of Bonar score were analysed separately, tenocyte morphology, collogen, and ground substance scores were statistically lower than the control group (p = 0.03, 0.041, 0.049, respectively). Vascularity parameter did not show any statistical difference (p > 0.05). Of the nine biomechanical parameters, five of them (failure load, cross-sectional area, length, ultimate stress, strain) showed better results which were also statistically significant (p = 0.046, 0.027, 0.011, 0.021, 0.002, respectively). When the remaining four parameters were examined, the study group also had better results, but this difference was not statistically significant. CONCLUSION: Curcumin had better results for total tendon healing not only histologically but also biomechanically. Curcumin could be an additional agent in the management of surgically repaired tendon injuries.
PURPOSE: This in vivo study aims to investigate the effects of curcumin which is recently developed for tendon healing using a ratAchilles tendon injury model. MATERIALS AND METHODS: Eighteen male Wistar albino rats weighing 300-400 g were used in this study. Under anesthesia, Achilles tendon injuries were created and repaired surgically. Nine rats of the study group received curcumin (suspended in saline at a dose of 200 mg/kg orally) and eight rats of the control group received only saline solution by oral gavage for a period of 28 days. Animals were euthanized on the 28th post-operative day, and all the Achilles tendons were removed and transferred immediately for biomechanic and histological analysis. RESULTS: Macroscopically, all the tendons were fully healed. Total mean Bonar score was higher in the control group. When the parameters of Bonar score were analysed separately, tenocyte morphology, collogen, and ground substance scores were statistically lower than the control group (p = 0.03, 0.041, 0.049, respectively). Vascularity parameter did not show any statistical difference (p > 0.05). Of the nine biomechanical parameters, five of them (failure load, cross-sectional area, length, ultimate stress, strain) showed better results which were also statistically significant (p = 0.046, 0.027, 0.011, 0.021, 0.002, respectively). When the remaining four parameters were examined, the study group also had better results, but this difference was not statistically significant. CONCLUSION:Curcumin had better results for total tendon healing not only histologically but also biomechanically. Curcumin could be an additional agent in the management of surgically repaired tendon injuries.
Authors: Mustafa Kulac; Cevat Aktas; Feti Tulubas; Ramazan Uygur; Mehmet Kanter; Mustafa Erboga; Mehmet Ceber; Birol Topcu; Oguz Aslan Ozen Journal: J Mol Histol Date: 2012-10-02 Impact factor: 2.611
Authors: Weizhong Zhang; Xuanyi Li; Mauro Comes Franchini; Ke Xu; Erica Locatelli; Robert C Martin; Ilaria Monaco; Yan Li; Shusen Cui Journal: Int J Nanomedicine Date: 2016-06-22