K Höglund1, J Häggström2, S Hanås3, A-C Merveille4, V Gouni5, M Wiberg6, J Lundgren Willesen7, K Mc Entee8, L Mejer Sørensen7, L Tiret9, E H Seppälä10, H Lohi10, V Chetboul5, M Fredholm7, A-S Lequarré4, I Ljungvall2. 1. Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Science, Uppsala, Sweden. Electronic address: Katja.Hoglund@slu.se. 2. Department of Clinical Sciences, Swedish University of Agricultural Science, Uppsala, Sweden. 3. Department of Clinical Sciences, Swedish University of Agricultural Science, Uppsala, Sweden; Evidensia Animal Clinic, 723 41 Västerås, Sweden. 4. Department of Clinical Sciences, University of Liège, Belgium. 5. Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, Unité de Cardiologie d'Alfort, Maisons-Alfort Cedex, France; Inserm, U955, Equipe 3, Créteil Cedex, France. 6. Department of Equine and Small Animal Medicine, University of Helsinki, Finland. 7. Department of Veterinary Clinical Sciences, University of Copenhagen, Denmark. 8. Department of Clinical Sciences, University of Liège, Belgium; Laboratory of Physiology and Pharmacology, Université Libre de Bruxelles, Belgium. 9. U955 IMRB, Team 10, Inserm, Ecole nationale vétérinaire d'Alfort, UPEC, Maisons-Alfort, France. 10. Research Programs Unit, Molecular Neurology, University of Helsinki and Folkhälsan Institute of Genetics, Finland.
Abstract
INTRODUCTION: Serotonin (5-hydroxytryptamine [5-HT]) has several biological functions. In different species, excessive 5-HT has been linked to valvular lesions, similar to those seen in dogs with myxomatous mitral valve disease. Previous studies suggest higher 5-HT in healthy Cavalier King Charles Spaniels (CKCSs), a breed highly affected by myxomatous mitral valve disease, compared to other breeds. OBJECTIVE: To investigate potential interbreed variation in serum 5-HT in healthy dogs. ANIMALS: 483 healthy dogs of nine breeds aged 1-7 years. METHODS: Dogs were examined at five European centers. Absence of cardiovascular, organ-related, or systemic diseases was ensured by thorough clinical investigations including echocardiography. Serum was frozen and later analyzed by enzyme-linked immunosorbent assay (ELISA). RESULTS: Median 5-HT concentration was 252.5 (interquartile range = 145.5-390.6) ng/mL. Overall breed difference was found (p<0.0001), and 42% of pairwise breed comparisons were significant. Univariate regression analysis showed association between serum 5-HT concentration and breed, center of examination, storage time, and sex, with higher 5-HT in females. In multiple regression analysis, the final model had an adjusted R2 of 0.27 with breed (p<0.0001), center (p<0.0001), and storage time (p=0.014) remaining significant. Within centers, overall breed differences were found at 3/5 centers (p≤0.028), and pairwise comparisons within those centers showed breed differences in 42% of comparisons. Among the included breeds, Newfoundlands, Belgian Shepherds and CKCSs had highest 5-HT concentrations. CONCLUSIONS: Interbreed variation in serum 5-HT concentration was found in healthy dogs aged 1-7 years. These differences should be taken into account when designing clinical studies.
INTRODUCTION:Serotonin (5-hydroxytryptamine [5-HT]) has several biological functions. In different species, excessive 5-HT has been linked to valvular lesions, similar to those seen in dogs with myxomatous mitral valve disease. Previous studies suggest higher 5-HT in healthy Cavalier King Charles Spaniels (CKCSs), a breed highly affected by myxomatous mitral valve disease, compared to other breeds. OBJECTIVE: To investigate potential interbreed variation in serum 5-HT in healthy dogs. ANIMALS: 483 healthy dogs of nine breeds aged 1-7 years. METHODS:Dogs were examined at five European centers. Absence of cardiovascular, organ-related, or systemic diseases was ensured by thorough clinical investigations including echocardiography. Serum was frozen and later analyzed by enzyme-linked immunosorbent assay (ELISA). RESULTS: Median 5-HT concentration was 252.5 (interquartile range = 145.5-390.6) ng/mL. Overall breed difference was found (p<0.0001), and 42% of pairwise breed comparisons were significant. Univariate regression analysis showed association between serum 5-HT concentration and breed, center of examination, storage time, and sex, with higher 5-HT in females. In multiple regression analysis, the final model had an adjusted R2 of 0.27 with breed (p<0.0001), center (p<0.0001), and storage time (p=0.014) remaining significant. Within centers, overall breed differences were found at 3/5 centers (p≤0.028), and pairwise comparisons within those centers showed breed differences in 42% of comparisons. Among the included breeds, Newfoundlands, Belgian Shepherds and CKCSs had highest 5-HT concentrations. CONCLUSIONS: Interbreed variation in serum 5-HT concentration was found in healthy dogs aged 1-7 years. These differences should be taken into account when designing clinical studies.
Authors: Mark A Oyama; Chad Elliott; Kerry A Loughran; Alexander P Kossar; Estibaliz Castillero; Robert J Levy; Giovanni Ferrari Journal: Cardiovasc Pathol Date: 2020-01-07 Impact factor: 2.185
Authors: Maria J Reimann; Merete Fredholm; Signe E Cremer; Liselotte B Christiansen; Kathryn M Meurs; Jacob E Møller; Jens Häggström; Jens Lykkesfeldt; Lisbeth H Olsen Journal: J Vet Intern Med Date: 2021-10-02 Impact factor: 3.333