Background: The pathogenesis of respiratory syncytial virus (RSV) in older adults may be due to age-related T-cell immunosenescence. Thus, we evaluated CD4 and CD8 T-cell responses during RSV infection in adults across the age spectrum. Methods: Peripheral blood mononuclear cells collected during RSV infection in adults, age 26-96 years, were stimulated with live RSV and peptide pools representing F, M, NP, and G proteins and analyzed by flow cytometry. Results: There were no significant age-related differences in frequency of CD4+ T cells synthesizing interferon (IFN)γ, interleukin (IL)2, IL4, IL10, or tumor necrosis factor (TNF)α or in CD8+IFNγ+ T cells. IL4+CD4+ T-cell numbers were low, as were IL13 and IL17 responses. However, in univariate analysis, CD4 T-cell IFNγ, IL2, IL4, IL10, and TNFα responses and CD8+IFNγ+ T cells were significantly increased with more severe illness requiring hospitalization. In multivariate analysis, viral load was also associated with increased T-cell responses. Conclusions: We found no evidence of diminished RSV-specific CD4 or CD8 T-cell responses in adults infected with RSV. However, adults with severe disease seemed to have more robust CD4 and CD8 T-cell responses during infection, suggesting that disease severity may have a greater association with T-cell responses than age.
Background: The pathogenesis of respiratory syncytial virus (RSV) in older adults may be due to age-related T-cell immunosenescence. Thus, we evaluated CD4 and CD8 T-cell responses during RSV infection in adults across the age spectrum. Methods: Peripheral blood mononuclear cells collected during RSV infection in adults, age 26-96 years, were stimulated with live RSV and peptide pools representing F, M, NP, and G proteins and analyzed by flow cytometry. Results: There were no significant age-related differences in frequency of CD4+ T cells synthesizing interferon (IFN)γ, interleukin (IL)2, IL4, IL10, or tumor necrosis factor (TNF)α or in CD8+IFNγ+ T cells. IL4+CD4+ T-cell numbers were low, as were IL13 and IL17 responses. However, in univariate analysis, CD4 T-cell IFNγ, IL2, IL4, IL10, and TNFα responses and CD8+IFNγ+ T cells were significantly increased with more severe illness requiring hospitalization. In multivariate analysis, viral load was also associated with increased T-cell responses. Conclusions: We found no evidence of diminished RSV-specific CD4 or CD8 T-cell responses in adults infected with RSV. However, adults with severe disease seemed to have more robust CD4 and CD8 T-cell responses during infection, suggesting that disease severity may have a greater association with T-cell responses than age.
Authors: Edward E Walsh; Derick R Peterson; Aja E Kalkanoglu; Frances Eun-Hyung Lee; Ann R Falsey Journal: J Infect Dis Date: 2013-02-04 Impact factor: 5.226
Authors: C B Hall; K R Powell; N E MacDonald; C L Gala; M E Menegus; S C Suffin; H J Cohen Journal: N Engl J Med Date: 1986-07-10 Impact factor: 91.245
Authors: Kyle Widmer; Marie R Griffin; Yuwei Zhu; John V Williams; H Keipp Talbot Journal: Influenza Other Respir Viruses Date: 2014-02-07 Impact factor: 4.380
Authors: Xin Hu; Ki-Hye Kim; Youri Lee; Jolyn Fernandes; M Ryan Smith; Yu-Jin Jung; Michael Orr; Sang-Moo Kang; Dean P Jones; Young-Mi Go Journal: Am J Pathol Date: 2019-05-18 Impact factor: 4.307
Authors: N C Salisch; A Izquierdo Gil; D N Czapska-Casey; L Vorthoren; J Serroyen; J Tolboom; E Saeland; H Schuitemaker; R C Zahn Journal: NPJ Vaccines Date: 2019-12-20 Impact factor: 7.344
Authors: Edward E Walsh; Thomas J Mariani; ChinYi Chu; Alex Grier; Steven R Gill; Xing Qiu; Lu Wang; Jeanne Holden-Wiltse; Anthony Corbett; Juilee Thakar; Matthew N McCall; David J Topham; Ann R Falsey; Mary T Caserta; Lauren Benoodt Journal: JMIR Res Protoc Date: 2019-06-06
Authors: N C Salisch; A Izquierdo Gil; D N Czapska-Casey; L Vorthoren; J Serroyen; J Tolboom; E Saeland; H Schuitemaker; R C Zahn Journal: NPJ Vaccines Date: 2019-12-20 Impact factor: 7.344