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Literature DB >> 2991916

In vitro mutagenesis of the v-sis transforming gene defines functional domains of its growth factor-related product.

C R King, N A Giese, K C Robbins, S A Aaronson.

Abstract

The polypeptide sequence of the v-sis transforming gene product of simian sarcoma virus (SSV) can be divided into four regions that are likely to represent structural domains of the protein. Mutations were generated in the SSV nucleotide sequence to assay the extent or function of each of these regions. The results indicate that the helper virus-derived amino-terminal sequence as well as a core region homologous to polypeptide chain 2 of platelet-derived growth factor (PDGF) are required for the transforming function of the protein. Products of transforming but not nontransforming mutants formed dimer structures conformationally analogous to biologically active PDGF.

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Year: 1985 PMID： 2991916 PMCID： PMC390554 DOI： 10.1073/pnas.82.16.5295

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

21 in total

1. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.

Authors: M Wigler; S Silverstein; L S Lee; A Pellicer; Y c Cheng; R Axel
Journal: Cell Date: 1977-05 Impact factor: 41.582

2. Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.

Authors: S L Adams; M E Sobel; B H Howard; K Olden; K M Yamada; B de Crombrugghe; I Pastan
Journal: Proc Natl Acad Sci U S A Date: 1977-08 Impact factor: 11.205

3. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors: G M Wahl; M Stern; G R Stark
Journal: Proc Natl Acad Sci U S A Date: 1979-08 Impact factor: 11.205

4. A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors: F L Graham; A J van der Eb
Journal: Virology Date: 1973-04 Impact factor: 3.616

5. Intracellular protein topogenesis.

Authors: G Blobel
Journal: Proc Natl Acad Sci U S A Date: 1980-03 Impact factor: 11.205

6. Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis.

Authors: S G Devare; E P Reddy; J D Law; K C Robbins; S A Aaronson
Journal: Proc Natl Acad Sci U S A Date: 1983-02 Impact factor: 11.205

7. In vivo identification of the transforming gene product of simian sarcoma virus.

Authors: K C Robbins; S G Devare; E P Reddy; S A Aaronson
Journal: Science Date: 1982-12-10 Impact factor: 47.728

8. Nucleotide sequence of the transforming gene of simian sarcoma virus.

Authors: S G Devare; E P Reddy; K C Robbins; P R Andersen; S R Tronick; S A Aaronson
Journal: Proc Natl Acad Sci U S A Date: 1982-05 Impact factor: 11.205

9. Biochemical and immunological characterization of polyproteins coded for by the McDonough, Gardner-Arnstein, and Snyder-Theilen strains of feline sarcoma virus.

Authors: M Barbacid; A V Lauver; S G Devare
Journal: J Virol Date: 1980-01 Impact factor: 5.103

10. Simian sarcoma virus onc gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor.

Authors: R F Doolittle; M W Hunkapiller; L E Hood; S G Devare; K C Robbins; S A Aaronson; H N Antoniades
Journal: Science Date: 1983-07-15 Impact factor: 47.728

14 in total

1. Recent developments in the structure, function and regulation of platelet-derived growth factor and its receptors.

Authors: J Tiesman; A Rizzino
Journal: Cytotechnology Date: 1989-12 Impact factor: 2.058

2. Immunochemical localization of the epitope for a monoclonal antibody that neutralizes human platelet-derived growth factor mitogenic activity.

Authors: W J LaRochelle; K C Robbins; S A Aaronson
Journal: Mol Cell Biol Date: 1989-08 Impact factor: 4.272

3. Deletions in the N-terminal coding region of the v-sis gene: determination of the minimal transforming region.

Authors: M K Sauer; M Hannink; D J Donoghue
Journal: J Virol Date: 1986-08 Impact factor: 5.103

4. Genetic organization of the c-sis transcription unit.

Authors: A M Van den Ouweland; J J Van Groningen; J A Schalken; H W Van Neck; H P Bloemers; W J Van de Ven
Journal: Nucleic Acids Res Date: 1987-02-11 Impact factor: 16.971

5. Identification of nonessential disulfide bonds and altered conformations in the v-sis protein, a homolog of the B chain of platelet-derived growth factor.

Authors: M K Sauer; D J Donoghue
Journal: Mol Cell Biol Date: 1988-03 Impact factor: 4.272

6. A small v-sis/platelet-derived growth factor (PDGF) B-protein domain in which subtle conformational changes abrogate PDGF receptor interaction and transforming activity.

Authors: N Giese; W J LaRochelle; M May-Siroff; K C Robbins; S A Aaronson
Journal: Mol Cell Biol Date: 1990-10 Impact factor: 4.272

7. Deletions in the C-terminal coding region of the v-sis gene: dimerization is required for transformation.

Authors: M Hannink; M K Sauer; D J Donoghue
Journal: Mol Cell Biol Date: 1986-04 Impact factor: 4.272

Review 8. Platelet-derived growth factor as a mediator of normal and neoplastic cell proliferation.

Authors: B Westermark; C H Heldin
Journal: Med Oncol Tumor Pharmacother Date: 1986

9. Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins.

Authors: F Rorsman; M Bywater; T J Knott; J Scott; C Betsholtz
Journal: Mol Cell Biol Date: 1988-02 Impact factor: 4.272

10. The phenotypic characteristics of simian sarcoma virus-transformed human fibroblasts suggest that the v-sis gene product acts solely as a PDGF receptor agonist in cell transformation.

Authors: A Johnsson; C Betsholtz; C H Heldin; B Westermark
Journal: EMBO J Date: 1986-07 Impact factor: 11.598