| Literature DB >> 29916070 |
Inês Carqueijeiro1, Liuda J Sepúlveda1,2, Angela Mosquera1,2, Richard Payne3, Cyrielle Corbin1, Nicolas Papon4, Thomas Dugé de Bernonville1, Sébastien Besseau1, Arnaud Lanoue1, Gaëlle Glévarec1, Marc Clastre1, Benoit St-Pierre1, Lucia Atehortùa2, Nathalie Giglioli-Guivarc'h1, Sarah E O'Connor3, Audrey Oudin1, Vincent Courdavault5.
Abstract
Accurate and efficient demonstrations of protein localizations to the vacuole or tonoplast remain strict prerequisites to decipher the role of vacuoles in the whole plant cell biology and notably in defence processes. In this chapter, we describe a reliable procedure of protein subcellular localization study through transient transformations of Catharanthus roseus or onion cells and expression of fusions with fluorescent proteins allowing minimizing artefacts of targeting.Entities:
Keywords: Allium cepa; Biolistic; Catharanthus roseus; GFP; Onion peel; Protein localization; Tonoplast; Transient transformation; Vacuolar targeting; Vacuole; YFP
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Year: 2018 PMID： 29916070 DOI： 10.1007/978-1-4939-7856-4_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745