I Sanz-Martín1,2, E Rojo3, E Maldonado4, G Stroppa3, J Nart3, M Sanz4. 1. Section of Periodontology, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain. ignaciosanzmartin@odon.ucm.es. 2. Department of Periodontology, Universitat Internacional de Catalunya (UIC), Barcelona, Spain. ignaciosanzmartin@odon.ucm.es. 3. Department of Periodontology, Universitat Internacional de Catalunya (UIC), Barcelona, Spain. 4. Section of Periodontology, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain.
Abstract
Tuberosity grafts had a greater percentage of lamina propria and lower percentage of submucosa when compared to lateral palate grafts. OBJECTIVE: The study aims to understand the differences in the structural composition of soft tissue autografts harvested from the lateral palate or the tuberosity. MATERIAL AND METHODS: Patients were randomly allocated to receive autografts harvested either from palatal or tuberosity sites to augment horizontal volume deficiencies around single-tooth implants. Tissue biopsies were analyzed for histological and histo-morphometric analysis. Picro-sirius red stain was used to evaluate collagen 1 and 3. Also, immuno-histochemical analysis was performed against MMP1, MMP2, cytokeratin-10, cytokeratin-13, and lysine hydroxylase-2. RESULTS:Twenty specimens were harvested from 9 subjects in the lateral palate group (PG) and 11 subjects in the tuberosity group (TG). The percentage of lamina propria represented 51.08% in the PG group and 72.79% in the TG group, while the area of submucosa was minimal in the TG group representing 4.89% of the total sample vs 25.75% in the PG. The total area of COL-1 and 3 in the TG was 1.19 ± 0.57 and 0.72 ± 0.44 mm2, respectively, while in the PG, the corresponding values were 1.4 ± 0.7 and 1.04 ± 0.5 mm2. The immuno-histochemical analysis generally showed a higher expression of LLH-2, MMP2, CYT-10, and CYT-13 in the TG when compared with the PG. CONCLUSION: Tuberosity grafts had a greater percentage of lamina propria and lower percentage of submucosa. The collagen content in the lamina propria was similar for both groups while the immuno-histochemical profile showed differences in the antibody expression of the epithelial cells. CLINICAL RELEVANCE: Tuberosity grafts had more lamina propria and less submocusa, which may be beneficial for volume augmentation.
RCT Entities:
Tuberosity grafts had a greater percentage of lamina propria and lower percentage of submucosa when compared to lateral palate grafts. OBJECTIVE: The study aims to understand the differences in the structural composition of soft tissue autografts harvested from the lateral palate or the tuberosity. MATERIAL AND METHODS:Patients were randomly allocated to receive autografts harvested either from palatal or tuberosity sites to augment horizontal volume deficiencies around single-tooth implants. Tissue biopsies were analyzed for histological and histo-morphometric analysis. Picro-sirius red stain was used to evaluate collagen 1 and 3. Also, immuno-histochemical analysis was performed against MMP1, MMP2, cytokeratin-10, cytokeratin-13, and lysine hydroxylase-2. RESULTS: Twenty specimens were harvested from 9 subjects in the lateral palate group (PG) and 11 subjects in the tuberosity group (TG). The percentage of lamina propria represented 51.08% in the PG group and 72.79% in the TG group, while the area of submucosa was minimal in the TG group representing 4.89% of the total sample vs 25.75% in the PG. The total area of COL-1 and 3 in the TG was 1.19 ± 0.57 and 0.72 ± 0.44 mm2, respectively, while in the PG, the corresponding values were 1.4 ± 0.7 and 1.04 ± 0.5 mm2. The immuno-histochemical analysis generally showed a higher expression of LLH-2, MMP2, CYT-10, and CYT-13 in the TG when compared with the PG. CONCLUSION: Tuberosity grafts had a greater percentage of lamina propria and lower percentage of submucosa. The collagen content in the lamina propria was similar for both groups while the immuno-histochemical profile showed differences in the antibody expression of the epithelial cells. CLINICAL RELEVANCE: Tuberosity grafts had more lamina propria and less submocusa, which may be beneficial for volume augmentation.
Authors: Kristina Bertl; Markus Pifl; Lena Hirtler; Barbara Rendl; Sylvia Nürnberger; Andreas Stavropoulos; Christian Ulm Journal: J Periodontol Date: 2015-08-20 Impact factor: 6.993
Authors: Ernest Rojo; Giorgio Stroppa; Ignacio Sanz-Martin; Oscar Gonzalez-Martín; Antonio Santos Alemany; José Nart Journal: J Clin Periodontol Date: 2018-02-23 Impact factor: 8.728
Authors: Franz J Strauss; Brandon J Huber; Ana Valdés; Ronald E Jung; Sven Mühlemann; Daniel S Thoma Journal: Clin Oral Investig Date: 2022-07-01 Impact factor: 3.606