Literature DB >> 29914956

CRISPR/Cas9 Genome Editing To Demonstrate the Contribution of Cyp51A Gly138Ser to Azole Resistance in Aspergillus fumigatus.

Takashi Umeyama1, Yuta Hayashi2, Hisaki Shimosaka3, Tatsuya Inukai4, Satoshi Yamagoe4, Shogo Takatsuka4, Yasutaka Hoshino4, Minoru Nagi4, Shigeki Nakamura4, Katsuhiko Kamei5, Kenji Ogawa2, Yoshitsugu Miyazaki4.   

Abstract

A pan-azole-resistant Aspergillus fumigatus strain with the cyp51A mutations Gly138Ser and Asn248Lys was isolated from a patient receiving long-term voriconazole treatment. PCR fragments containing cyp51A with the mutations were introduced along with the Cas9 protein and single guide RNA into the azole-resistant/susceptible strains. Recombinant strains showed increased susceptibility via the replacement of Ser138 by glycine. Genetic recombination, which has been hampered thus far in clinical isolates, can now be achieved using CRISPR/Cas9 genome editing.
Copyright © 2018 American Society for Microbiology.

Entities:  

Keywords:  CRISPR; Cas9; Cyp51A; antifungal resistance; azole drugs; genome editing

Mesh：

Substances：

Year:  2018        PMID： 29914956      PMCID： PMC6125507          DOI： 10.1128/AAC.00894-18

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  20 in total

1.  Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity.

Authors:  F Ann Ran; Patrick D Hsu; Chie-Yu Lin; Jonathan S Gootenberg; Silvana Konermann; Alexandro E Trevino; David A Scott; Azusa Inoue; Shogo Matoba; Yi Zhang; Feng Zhang
Journal:  Cell       Date:  2013-08-29       Impact factor: 41.582

2.  Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus.

Authors:  Kevin K Fuller; Shan Chen; Jennifer J Loros; Jay C Dunlap
Journal:  Eukaryot Cell       Date:  2015-08-28

Review 3.  Genome editing. The new frontier of genome engineering with CRISPR-Cas9.

Authors:  Jennifer A Doudna; Emmanuelle Charpentier
Journal:  Science       Date:  2014-11-28       Impact factor: 47.728

Review 4.  Executive Summary of Japanese Domestic Guidelines for Management of Deep-seated Mycosis 2014.

Authors:  Shigeru Kohno; Kazuo Tamura; Yoshihito Niki; Koichi Izumikawa; Shinichi Oka; Kenji Ogawa; Junichi Kadota; Katsuhiko Kamei; Yoshinobu Kanda; Tetsuya Kiuchi; Kazutoshi Shibuya; Syunji Takakura; Toru Takata; Yoshio Takesue; Katsuji Teruya; Issei Tokimatsu; Takahiro Fukuda; Shigefumi Maesaki; Koichi Makimura; Hiroshige Mikamo; Kotaro Mitsutake; Yoshitsugu Miyazaki; Masaaki Mori; Akira Yasuoka; Keiko Yano; Noboru Yamanaka; Minoru Yoshida
Journal:  Med Mycol J       Date:  2016

5.  Genome-scale CRISPR-Cas9 knockout screening in human cells.

Authors:  Ophir Shalem; Neville E Sanjana; Ella Hartenian; Xi Shi; David A Scott; Tarjei Mikkelson; Dirk Heckl; Benjamin L Ebert; David E Root; John G Doench; Feng Zhang
Journal:  Science       Date:  2013-12-12       Impact factor: 47.728

Review 6.  Triazole resistance surveillance in Aspergillus fumigatus.

Authors:  Agustin Resendiz Sharpe; Katrien Lagrou; Jacques F Meis; Anuradha Chowdhary; Shawn R Lockhart; Paul E Verweij
Journal:  Med Mycol       Date:  2018-04-01       Impact factor: 4.076

7.  The structure-function relationship of the Aspergillus fumigatuscyp51A L98H conversion by site-directed mutagenesis: the mechanism of L98H azole resistance.

Authors:  Eveline Snelders; Anna Karawajczyk; Rob J A Verhoeven; Hanka Venselaar; Gijs Schaftenaar; Paul E Verweij; Willem J G Melchers
Journal:  Fungal Genet Biol       Date:  2011-08-30       Impact factor: 3.495

8.  Genotype-phenotype complexity of the TR46/Y121F/T289A cyp51A azole resistance mechanism in Aspergillus fumigatus.

Authors:  Eveline Snelders; Simone M T Camps; Anna Karawajczyk; Antonius J M M Rijs; Jan Zoll; Paul E Verweij; Willem J G Melchers
Journal:  Fungal Genet Biol       Date:  2015-06-16       Impact factor: 3.495

9.  Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus.

Authors:  Chi Zhang; Xiuhua Meng; Xiaolei Wei; Ling Lu
Journal:  Fungal Genet Biol       Date:  2015-12-14       Impact factor: 3.495

10.  A Simple and Universal System for Gene Manipulation in Aspergillus fumigatus: In Vitro-Assembled Cas9-Guide RNA Ribonucleoproteins Coupled with Microhomology Repair Templates.

Authors:  Qusai Al Abdallah; Wenbo Ge; Jarrod R Fortwendel
Journal:  mSphere       Date:  2017-11-22       Impact factor: 4.389
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  15 in total

1.  Aspergillus fumigatus Cyp51A and Cyp51B Proteins Are Compensatory in Function and Localize Differentially in Response to Antifungals and Cell Wall Inhibitors.

Authors:  Mark T Roundtree; Praveen R Juvvadi; E Keats Shwab; D Christopher Cole; William J Steinbach
Journal:  Antimicrob Agents Chemother       Date:  2020-09-21       Impact factor: 5.191

2.  A Simple Method To Detect Point Mutations in Aspergillus fumigatus cyp51A Gene Using a Surveyor Nuclease Assay.

Authors:  Teppei Arai; Hidetaka Majima; Akira Watanabe; Katsuhiko Kamei
Journal:  Antimicrob Agents Chemother       Date:  2020-03-24       Impact factor: 5.191

Review 3.  Aspergillus fumigatus and aspergillosis: From basics to clinics.

Authors:  A Arastehfar; A Carvalho; J Houbraken; L Lombardi; R Garcia-Rubio; J D Jenks; O Rivero-Menendez; R Aljohani; I D Jacobsen; J Berman; N Osherov; M T Hedayati; M Ilkit; D James-Armstrong; T Gabaldón; J Meletiadis; M Kostrzewa; W Pan; C Lass-Flörl; D S Perlin; M Hoenigl
Journal:  Stud Mycol       Date:  2021-05-10       Impact factor: 16.097

Review 4.  Aspergillus fumigatus and Aspergillosis in 2019.

Authors:  Jean-Paul Latgé; Georgios Chamilos
Journal:  Clin Microbiol Rev       Date:  2019-11-13       Impact factor: 26.132

5.  Dysfunction of Ras-GAP protein AfgapA contributes to hypoxia fitness in Aspergillus fumigatus.

Authors:  Cai Bian; Yoko Kusuya; Daisuke Hagiwara; Sayaka Ban; Yu Lu; Masaki Nagayama; Hiroki Takahashi
Journal:  Curr Genet       Date:  2022-08-08       Impact factor: 2.695

6.  A novel Zn2-Cys6 transcription factor clcA contributes to copper homeostasis in Aspergillus fumigatus.

Authors:  Yoko Kusuya; Cai Bian; Daisuke Hagiwara; Sayaka Ban; Hiroki Takahashi
Journal:  Curr Genet       Date:  2022-08-16       Impact factor: 2.695

Review 7.  Ten decadal advances in fungal biology leading towards human well-being.

Authors:  Ausana Mapook; Kevin D Hyde; Khadija Hassan; Blondelle Matio Kemkuignou; Adéla Čmoková; Frank Surup; Eric Kuhnert; Pathompong Paomephan; Tian Cheng; Sybren de Hoog; Yinggai Song; Ruvishika S Jayawardena; Abdullah M S Al-Hatmi; Tokameh Mahmoudi; Nadia Ponts; Lena Studt-Reinhold; Florence Richard-Forget; K W Thilini Chethana; Dulanjalee L Harishchandra; Peter E Mortimer; Huili Li; Saisamorm Lumyong; Worawoot Aiduang; Jaturong Kumla; Nakarin Suwannarach; Chitrabhanu S Bhunjun; Feng-Ming Yu; Qi Zhao; Doug Schaefer; Marc Stadler
Journal:  Fungal Divers       Date:  2022-09-15       Impact factor: 24.902

Review 8.  The Fungal CYP51s: Their Functions, Structures, Related Drug Resistance, and Inhibitors.

Authors:  Jingxiang Zhang; Liping Li; Quanzhen Lv; Lan Yan; Yan Wang; Yuanying Jiang
Journal:  Front Microbiol       Date:  2019-04-24       Impact factor: 5.640

9.  Mutations in hmg1, Challenging the Paradigm of Clinical Triazole Resistance in Aspergillus fumigatus.

Authors:  P David Rogers; Jarrod R Fortwendel; Jeffrey M Rybak; Wenbo Ge; Nathan P Wiederhold; Josie E Parker; Steven L Kelly
Journal:  mBio       Date:  2019-04-02       Impact factor: 7.867

Review 10.  Advances in Molecular Tools and In Vivo Models for the Study of Human Fungal Pathogenesis.

Authors:  Dhara Malavia; Neil A R Gow; Jane Usher
Journal:  Microorganisms       Date:  2020-05-26
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