| Literature DB >> 29912628 |
Cuiling Lu1, Hongbin Chi1, Yapeng Wang1, Xue Feng1, Lina Wang1, Shuo Huang1, Liying Yan1, Shengli Lin1, Ping Liu1, Jie Qiao1.
Abstract
In an attempt to explore the early developmental arrest in embryos from polycystic ovarian syndrome (PCOS) patients, we sequenced the transcriptome profiles of PCOS arrested 2-cell embryos, non-PCOS arrested 2-cell embryos and non-arrested 2-cell embryos using single-cell RNA-Seq technique. Differential expression analysis was performed using the DEGSeq R package. Gene Ontology (GO) enrichment was analyzed using the GOseq R package. Data revealed 62 differentially expressed genes between non-PCOS arrested and PCOS arrested embryos and 2217 differentially expressed genes between PCOS arrested and non-arrested 2-cell embryos. A total of 49 differently expressed genes (DEGs) were annotated with GO terms in the up-regulated genes between PCOS arrested and non-PCOS arrested embryos after GO enrichment. A total of 29 DEGs were annotated with GO terms in the down-regulated genes between PCOS arrested and non-arrested 2-cell embryos after GO enrichment. These data can provide a reference for screening specific genes involved in the arrest of PCOS embryos.Entities:
Keywords: PCOS; embryo arrest; transcriptome
Mesh：
Year: 2018 PMID： 29912628 PMCID： PMC6103702 DOI： 10.1080/15384101.2018.1467678
Source DB: PubMed Journal: Cell Cycle ISSN： 1551-4005 Impact factor: 4.534