Literature DB >> 29906704

Conserved cysteines in Mason-Pfizer monkey virus capsid protein are essential for infectious mature particle formation.

Růžena Píchalová1, Tibor Füzik2, Barbora Vokatá3, Michaela Rumlová4, Manuel Llano5, Alžběta Dostálková6, Ivana Křížová7, Tomáš Ruml8, Pavel Ulbrich9.   

Abstract

Retrovirus assembly is driven mostly by Gag polyprotein oligomerization, which is mediated by inter and intra protein-protein interactions among its capsid (CA) domains. Mason-Pfizer monkey virus (M-PMV) CA contains three cysteines (C82, C193 and C213), where the latter two are highly conserved among most retroviruses. To determine the importance of these cysteines, we introduced mutations of these residues in both bacterial and proviral vectors and studied their impact on the M-PMV life cycle. These studies revealed that the presence of both conserved cysteines of M-PMV CA is necessary for both proper assembly and virus infectivity. Our findings suggest a crucial role of these cysteines in the formation of infectious mature particles.
Copyright © 2018. Published by Elsevier Inc.

Entities:  

Keywords:  Cysteine mutagenesis; M-PMV capsid; M-PMV infectivity; Retrovirus assembly; Virus core stability

Mesh：

Substances：

Year:  2018        PMID： 29906704      PMCID： PMC6379149          DOI： 10.1016/j.virol.2018.06.001

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  55 in total

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Authors:  D K Worthylake; H Wang; S Yoo; W I Sundquist; C P Hill
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-01-01

2.  Balancing selection and the evolution of functional polymorphism in Old World monkey TRIM5alpha.

Authors:  Ruchi M Newman; Laura Hall; Michelle Connole; Guo-Lin Chen; Shuji Sato; Eloisa Yuste; William Diehl; Eric Hunter; Amitinder Kaur; Gregory M Miller; Welkin E Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  2006-12-01       Impact factor: 11.205

3.  Distinct roles for nucleic acid in in vitro assembly of purified Mason-Pfizer monkey virus CANC proteins.

Authors:  Pavel Ulbrich; Sarka Haubova; Milan V Nermut; Eric Hunter; Michaela Rumlova; Tomas Ruml
Journal:  J Virol       Date:  2006-07       Impact factor: 5.103

4.  In vitro assembly properties of purified bacterially expressed capsid proteins of human immunodeficiency virus.

Authors:  I Gross; H Hohenberg; H G Kräusslich
Journal:  Eur J Biochem       Date:  1997-10-15

5.  Purification of proteins containing zinc finger domains using immobilized metal ion affinity chromatography.

Authors:  Irena Voráčková; Sárka Suchanová; Pavel Ulbrich; William E Diehl; Tomáš Ruml
Journal:  Protein Expr Purif       Date:  2011-05-10       Impact factor: 1.650

6.  Analysis of Mason-Pfizer monkey virus Gag domains required for capsid assembly in bacteria: role of the N-terminal proline residue of CA in directing particle shape.

Authors:  M Rumlova-Klikova; E Hunter; M V Nermut; I Pichova; T Ruml
Journal:  J Virol       Date:  2000-09       Impact factor: 5.103

7.  In vitro assembly of feline immunodeficiency virus capsid protein: biological role of conserved cysteines.

Authors:  M D Nath; D L Peterson
Journal:  Arch Biochem Biophys       Date:  2001-08-15       Impact factor: 4.013

8.  SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins.

Authors:  Michael P Malakhov; Michael R Mattern; Oxana A Malakhova; Mark Drinker; Stephen D Weeks; Tauseef R Butt
Journal:  J Struct Funct Genomics       Date:  2004

9.  The effect of point mutations within the N-terminal domain of Mason-Pfizer monkey virus capsid protein on virus core assembly and infectivity.

Authors:  Marcela Wildová; Romana Hadravová; Jitka Stokrová; Ivana Krízová; Tomás Ruml; Eric Hunter; Iva Pichová; Michaela Rumlová
Journal:  Virology       Date:  2008-08-27       Impact factor: 3.616

10.  Stabilization of the β-hairpin in Mason-Pfizer monkey virus capsid protein- a critical step for infectivity.

Authors:  Martin Obr; Romana Hadravová; Michal DoleŽal; Ivana KříŽová; Veronika Papoušková; Lukáš Zídek; Richard Hrabal; Tomáš Ruml; Michaela Rumlová
Journal:  Retrovirology       Date:  2014-10-30       Impact factor: 4.602
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  1 in total

1.  Fullerene Derivatives Prevent Packaging of Viral Genomic RNA into HIV-1 Particles by Binding Nucleocapsid Protein.

Authors:  Ivana Křížová; Alžběta Dostálková; Edison Castro; Jan Prchal; Romana Hadravová; Filip Kaufman; Richard Hrabal; Tomáš Ruml; Manuel Llano; Luis Echegoyen; Michaela Rumlová
Journal:  Viruses       Date:  2021-12-06       Impact factor: 5.048
  1 in total

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