Monitoring alcohol concentration and residual glucose in solid state fermentation of ethanol using FT-NIR spectroscopy and L1-PLS regression.

This study aimed to investigate the potential of FT-NIR spectroscopy technique combined with chemometrics method, which employed to monitor time-related changes of alcohol concentration and residual glucose during solid state fermentation (SSF) of ethanol. Characteristic wavelength variables were firstly selected by use of L1-norm regularization approach. Then, the partial least squares (PLS) regression model was finally developed using the variables selected by L1-norm regularization method to quantitative determine alcohol concentration and residual glucose in SSF of ethanol. Compared with the best results of full-spectrum PLS, the L1-PLS model obtained better results as follows: RMSECV = 1.0392 g/L, Rc = 0.9911, RMSEP = 1.0910 g/L, Rp = 0.9917 for alcohol concentration; RMSECV = 1.7002 g/L, Rc = 0.9880, RMSEP = 2.1859 g/L, Rp = 0.9896 for residual glucose. The overall results sufficiently demonstrate that FT-NIR spectroscopy technique coupled with appropriate chemometrics method is a promising tool for monitoring the process of SSF of ethanol.