OBJECTIVE: To explore the effect and mechanism of butyl phydroxybenzoate( BP) on sperm motility, oxidative stress and apoptosis. METHODS: Semen samples from 20 healthy sperm donors were randomly divided into four groups:group control, BP 200 μmol/L, BP 400 μmol/L and BP 800 μmol/L, each group had five parallel samples. The spermatozoa were cultured with BP for 4 h in vitro, with the exposed concentration of BP at 0, 200, 400 and 800 μmol/L, respectively. The influence of BP on spermatozoa were analyzed by sperm activity, cytotoxicity, the rate of reactive oxygen species( ROS) positive cell and apoptosis. RESULTS: The total sperm activity in group BP200 μmol/L, BP 400 μmol/L and BP 800 μmol/L were( 47. 67 ± 3. 93) %, ( 32. 79 ±2. 90) %, ( 10. 51 ± 5. 88) % respectively, which were significantly lower than control( 26. 44 ± 7. 83) %. Compared with the control group, the survival rate of sperm in the three experiment groups were( 63. 36 ± 9. 08) %, ( 49. 72 ± 7. 15) %, ( 29. 91 ±5. 93) % respectively. Rate of ROS positive cells in the three experiment groups were( 24. 67 ± 0. 50) %, ( 54. 50 ± 3. 40) %, ( 59. 93 ± 3. 47) % respectively, which were significantly higher than control( 8. 63 ± 0. 57) %. The rate of late stage apoptosis were( 11. 8 ± 1. 74) %, ( 12. 87 ± 0. 25) %, ( 14. 60 ± 0. 87) % respectively, which were significantly higher than control( 9. 63 ± 1. 00) %. The level of sperm ROS and the late stage apoptosis rate were negatively correlated with the total sperm motility, correlation coefficient were- 0. 727 and- 0. 688 respectively( P < 0. 05). CONCLUSION: BP has cytotoxicity and can reduce sperm motility and promote the production of sperm ROS.
RCT Entities:
OBJECTIVE: To explore the effect and mechanism of butyl phydroxybenzoate( BP) on sperm motility, oxidative stress and apoptosis. METHODS: Semen samples from 20 healthy sperm donors were randomly divided into four groups:group control, BP 200 μmol/L, BP 400 μmol/L and BP 800 μmol/L, each group had five parallel samples. The spermatozoa were cultured with BP for 4 h in vitro, with the exposed concentration of BP at 0, 200, 400 and 800 μmol/L, respectively. The influence of BP on spermatozoa were analyzed by sperm activity, cytotoxicity, the rate of reactive oxygen species( ROS) positive cell and apoptosis. RESULTS: The total sperm activity in group BP200 μmol/L, BP 400 μmol/L and BP 800 μmol/L were( 47. 67 ± 3. 93) %, ( 32. 79 ±2. 90) %, ( 10. 51 ± 5. 88) % respectively, which were significantly lower than control( 26. 44 ± 7. 83) %. Compared with the control group, the survival rate of sperm in the three experiment groups were( 63. 36 ± 9. 08) %, ( 49. 72 ± 7. 15) %, ( 29. 91 ±5. 93) % respectively. Rate of ROS positive cells in the three experiment groups were( 24. 67 ± 0. 50) %, ( 54. 50 ± 3. 40) %, ( 59. 93 ± 3. 47) % respectively, which were significantly higher than control( 8. 63 ± 0. 57) %. The rate of late stage apoptosis were( 11. 8 ± 1. 74) %, ( 12. 87 ± 0. 25) %, ( 14. 60 ± 0. 87) % respectively, which were significantly higher than control( 9. 63 ± 1. 00) %. The level of sperm ROS and the late stage apoptosis rate were negatively correlated with the total sperm motility, correlation coefficient were- 0. 727 and- 0. 688 respectively( P < 0. 05). CONCLUSION:BP has cytotoxicity and can reduce sperm motility and promote the production of sperm ROS.