Literature DB >> 2990254

Spectrophotometric assay for vertebrate collagenase.

H Weingarten, J Feder.   

Abstract

Collagenase from normal human skin fibroblasts was found to catalyze the hydrolysis of esters and thio esters. This observation led to the development of a rapid, sensitive, continuous spectrophotometric assay for vertebrate collagenase using the thio peptolide Ac-ProLeuGly-S-LeuLeuGly-OC2H5 as substrate in the presence of 4,4'-dithiodipyridine or Ellman's Reagent. A Km of 0.004 M and a kcat of 370,000 h-1 were determined for the thio peptolide-enzyme reaction. The method is able to detect collagenase at concentrations as low as 2 ng/ml.

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Year:  1985        PMID： 2990254     DOI： 10.1016/0003-2697(85)90294-5

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  Matrix metalloproteinase-10 (MMP-10) interaction with tissue inhibitors of metalloproteinases TIMP-1 and TIMP-2: binding studies and crystal structure.

Authors:  Jyotica Batra; Jessica Robinson; Alexei S Soares; Alan P Fields; Derek C Radisky; Evette S Radisky
Journal:  J Biol Chem       Date:  2012-03-16       Impact factor: 5.157

2.  Assignments, secondary structure and dynamics of the inhibitor-free catalytic fragment of human fibroblast collagenase.

Authors:  F J Moy; M R Pisano; P K Chanda; C Urbano; L M Killar; M L Sung; R Powers
Journal:  J Biomol NMR       Date:  1997-07       Impact factor: 2.835

3.  A new methodology for monitoring the activity of cdMMP-12 anchored and freeze-dried on Au (111).

Authors:  Giuseppe Grasso; Marco Fragai; Enrico Rizzarelli; Giuseppe Spoto; Kwon Joo Yeo
Journal:  J Am Soc Mass Spectrom       Date:  2007-03-23       Impact factor: 3.109

4.  N-alkyl urea hydroxamic acids as a new class of peptide deformylase inhibitors with antibacterial activity.

Authors:  Corinne J Hackbarth; Dawn Z Chen; Jason G Lewis; Kirk Clark; James B Mangold; Jeffrey A Cramer; Peter S Margolis; Wen Wang; Jim Koehn; Charlotte Wu; S Lopez; George Withers; Helen Gu; Elina Dunn; R Kulathila; Shi-Hao Pan; Wilma L Porter; Jeff Jacobs; Joaquim Trias; Dinesh V Patel; Beat Weidmann; Richard J White; Zhengyu Yuan
Journal:  Antimicrob Agents Chemother       Date:  2002-09       Impact factor: 5.191

5.  Photodynamic molecular beacon as an activatable photosensitizer based on protease-controlled singlet oxygen quenching and activation.

Authors:  Gang Zheng; Juan Chen; Klara Stefflova; Mark Jarvi; Hui Li; Brian C Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  2007-05-14       Impact factor: 11.205

6.  Matrix Metalloproteinase-3 (MMP-3) Is an Endogenous Activator of the MMP-9 Secreted by Placental Leukocytes: Implication in Human Labor.

Authors:  Arturo Flores-Pliego; Aurora Espejel-Nuñez; Marisol Castillo-Castrejon; Noemi Meraz-Cruz; Jorge Beltran-Montoya; Veronica Zaga-Clavellina; Sonia Nava-Salazar; Maribel Sanchez-Martinez; Felipe Vadillo-Ortega; Guadalupe Estrada-Gutierrez
Journal:  PLoS One       Date:  2015-12-29       Impact factor: 3.240
  6 in total

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