Preparation and evaluation of spirulina polysaccharide nanoemulsions.

The aim of the present study was to prepare spirulina polysaccharide (PSP) into an oral nanoemulsion (NE) with the aim of improving its oral bioavailability and prolonging its sustained release effect. The PSP‑NE was prepared through a phase transformation method, and its formulation components were screened through the use of a pseudo‑ternary phase diagram. The optimal formulation of PSP‑NE was determined to be: 11.9% Span 80, 6.0% Tween-80, 9.0% ethanol, 62.8% soybean oil, and 10.3% PSP aqueous solution. The prepared PSP‑NE was clear and transparent, had a uniform color and spherical morphology, exhibited stability and no adhesion. The average particle size was 79.93±19 nm, the polydispersity index was 0.185±0.04 (n=3), and the entrapment rate was 62%. Small‑animal imaging results showed that the prepared PSP‑NE exhibited a sustained release and tissue effect in contrast to the PSP aqueous solution. The present study showed that the prepared PSP‑NE not only exhibited a sustained release and tissue effect in contrast to the PSP aqueous solution, but also had superior performance in terms of antitumor and antioxidant effects.

Introduction

Spirulina polysaccharide (PSP), a type of water-soluble, physiologically active polysaccharide extracted from spirulina, has a large and complex molecular structure, which is mainly composed of glycosidic bonds (1). PSP is reported to have an effect on inhibiting tumor cell growth through inhibiting the synthesis of nucleic acid and proteins in cancer cells, but not directly killing cancer cells. In addition, the inhibitory effect of PSP on cancer cells has been reported to be time-dependent (2,3). It is well known that free radicals can oxidize biomolecules and are important in several degenerative and pathological processes (4,5). As an antioxidant, PSP can maintain cellular health and inhibit senescence in the body by removing excess free radicals and preventing the oxidation of cellular oxidative substrates (6,7). PSP can enhance the non-specific cellular immune function in the body, and improve the ability to resist the invasion of viruses (8). Therefore, it has potential application and development value.

Nanoemulsions (NEs) (9) are formed spontaneously by mixing together an aqueous phase, an oil phase, surfactant, and co-surfactant; can be a thermodynamically stable, isotropic, transparent, or translucent homogeneous dispersion; they have a particle size of 1–100 nm. NEs are stabilized and not layered by autoclaving and high-speed centrifugation (10). NEs have been used since 1930, particularly in floor polishing products, including liquid waxes, fuels and dry lotions (11). At present, NE technology is employed in the production of commonly used chemicals, specialized chemicals, and in the petroleum and materials science (12,13), biotechnology and pharmaceutical industries (14). Furthermore, NEs are novel drug carriers and offer numerous beneficial properties (15). In particular, they exhibit thermodynamic stability and low viscosity, and are thus easy to prepare and store. They also increase the solubility of fat-soluble drugs.

The pseudo-ternary phase diagram method is always applied to reflect the mutual change of a three-component system on a plane triangle under isothermal isobaric conditions. In NEs, the three vertices in the pseudo-ternary phase diagram respectively represent the aqueous phase, the oil phase, and the surfactant. The ratio between two components is determined by the distance from this point to the endpoints on both sides, and the proportional relationship between the three components is within the pseudo-ternary phase diagram. This method turns the association among the three components in the NE formation into a flat graph, which is intuitive, concise and enables easy determination of the proportion of each component.

Studies have indicated that a PSP-loaded NE offers a wide application in clinical use, including increasing stability and sustained drug release. Furthermore, a PSP-loaded NE has been shown to improve clinical medication safety by reducing its irritant or toxic side effects, enhancing its availability and bioavailability by avoiding destruction when passing through the digestive tract, and increasing specificity to drug targeting by extending the release time of water-soluble drugs. In addition to achieving the above effects, a PSP-loaded NE can mask strong odors to improve patient compliance (16,17). Therefore, the fish-flavored PSP was encapsulated into NE to prepare PSP-NE, which aimed to improve the oral bioavailability and stability of PSP, as well as the antitumor and antioxidative effects.

Materials and methods

Materials

The PSP (polysaccharide content of 71.65%) produced by the Department of Traditional Chinese Medicine Department of Jinan University (Guangzhou, China) was a spirulina lye extract product, and the monosaccharide composition was as follows: Glucose (21.3±1.4%), rhamnose (43.6±2.7%), xylose (2.4±0.6%), galactose (1.3±0.2%) and arabinose (1.1±0.1%). Other reagents included indocyanine green (MedChem Express, Monmouth Junction, NJ, USA), 1,9-dimethylmethylene blue (DMB Sigma; EMD Millipore, Billerica, MA, USA), Span 80 (Aladdin Reagent Co., Ltd., Shanghai, China), Tween-80 (Aladdin Reagent Co., Ltd.), injection-grade soybean oil (Emerging Tieling Pharmaceutical Co., Ltd., Tieling, China), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT; Sigma; EMD Millipore), RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific. Inc.) Sudan Red III, methylene blue, dimethyl sulfoxide, ethanol, iron sulfate, isopropanol, n-butanol, n-octanol and polyethylene glycol 400 (Tianjin Daming Chemical Reagent Factory, Tianjin, China).

Experimental animals and tumor cell line

A total of 9 BALB/c-nu mice (age: 8 weeks; weight: ~22 g) with equal numbers of males and females were provided by Beijing Huafu Kang Biotechnology Co., Ltd. (Beijing, China), and were maintained in our facility with free access to water and food, under a 12-h light/dark cycle, with 40% humidity. The HepG2 human hepatoblastoma cell line and MCF-7 human breast adenocarcinoma cell line were obtained from Jiangsu KeyGen Biotech Co., Ltd., (Jiangsu, China).

Surfactant preparation

The NEs were prepared with a high-speed homogenizer. For formulation screening, a surfactant or mixed surfactant was selected according to the NE type declared. A low hydrophile-lipophile balance (HLB) value (<8) was used for the preparation of a water-oil (W/O) NE and a high HLB value (>10) was used for an oil-water (O/W) NE. As PSP was readily soluble, the PSP was mixed with the water-in-oil NE.

Screening of PSP-NE components

Combination of surfactants

The effects of seven complexes of mixed surfactants (Span 80 and glycerolmonooleate at ratios of 3:2 and 3:4; glycerolmonooleate and Tween-80 at ratios of 2:1, 3:1 and 4:1, and Span 80/Tween-80 at ratios of 3:1 and 4:1) were investigated on the NE-forming area of a pseudo-ternary phase diagram in the surfactant/anhydrous ethanol/soybean oil/water system [surfactant and co-surfactant ratio (Km) of 2:1] at room temperature (18).

Screening of co-surfactants

At room temperature, the effects of five co-surfactants (ethanol, isopropanol, polyethylene glycol 400, n-butanol and n-octanol) on the NE-forming area of the pseudo-ternary phase diagram were investigated in a Span 80/Tween-80/co-surfactant/soybean oil/water system (Km 2:1).

Effect of Km on the formation of NEs

The effects of Km values at 2:1, 3:1 and 4:1 on the NE-forming area of the pseudo-ternary phase diagram were investigated in a Span 80/Tween-80/anhydrous ethanol/soybean oil/water system at room temperature.

Screening of oil phase

Liquid paraffin, medium-chain fatty acid triglyceride, soybean oil and sesame oil were used to determine whether they have individual effects on the NE-forming regions. This step was performed on a Span 80/Tween-80/anhydrous ethanol/oil phase/water (Km 2:1) system at room temperature.

Screening of the aqueous phase

The individual effects of 1, 2, 5, 10 and 20% PSP aqueous solutions on the NE-forming areas were investigated in a Span 80/Tween-80/anhydrous ethanol/soybean oil/PSP aqueous solution system (Km 2:1) at room temperature.

Titration to construct the pseudo-ternary phase diagram

The pseudo-ternary phase diagram was drawn by titration. For the determination of the optimal prescription as the main evaluation index for the NE formation, the mixed (Smix), oil and aqueous phases were used as the three vertices of the pseudo-ternary phase diagram on the basis of the size of the NE-forming area (19,20).

Evaluation of NE quality

Appearance and type

The appearance of the prepared NE with respect to clarity, transparency and homogeneity was examined macroscopically. The type of NE was determined by using different modes of the spreading speed of methylene blue (water-soluble dye) and Sudan red (oil-soluble dye) (21).

Shape and particle size

The shape was observed using transmission electron microscopy (TEM; Tecnai 10; Philips Healthcare, DA Best, The Netherlands) following diluting of the NE with soybean oil. The particle size and polydispersity index (PDI) of the prepared NE were measured with a laser particle size analyzer (Nano ZS 90; Malvern Instruments, Inc., Westborough, MA, USA) (22).

Viscosity and pH

The viscosities and pH values of the empty NE and PSP-NE were measured with a viscometer (SV-10 type; A&D Co., Ltd., Tokyo, Japan) and pH meter (pH 10/100 type, Shanghai Di Yim Instrument Co., Ltd., Shanghai, China), respectively.

Stability

The stability of the NEs were determined through a hot and cold cycle test (4 and 60°C), light experiment (4,500±500 lx) and accelerated experiment (40±2°C, 75±5% RH).

PSP content in the PSP-NE and entrapment rate

Following extraction from the prepared PSP-NE (1 ml) with a mixed solvent of chloroform and ethanol (1:1), an appropriate volume of acetone was added, and the PSP-NE was then subjected to ultrasonication until breakage. The PSP content in the prepared PSP-NE (C1) was measured by high-performance liquid chromatography (HPLC; Agilent 1260, Agilent Technologies, Inc., Santa, Clara, CA, USA) for refractive index (RI) detection.

The PSP-NE (1 ml) was placed in a 10 ml volumetric flask. An equal volume of acetone was then added. The PSP-NE was subjected to ultrasonication until it breakage and maintained constant at 10 ml. The total quantity of PSP (C2) was also measured by HPLC and RI detection. The entrapment rate was calculated according to the following formula: Entrapment rate (%)=C1/C2 ×100%.

Distribution of PSP-NE following oral administration

Indocyanine green was dissolved in the PSP aqueous solution (ICG-PSP) and then added to the NE to form an ICG-PSP-NE complex (The final concentration of ICG in both solutions is 0.05 mg/ml). A total of 9 BALB/C nude mice were assigned to each of three experimental groups according to treatment type (0.1 ml/100 g of ICG-PSP, ICG-PSP-NE, and physiological saline, ig). The mice were subjected to fasting 4 h following their respective treatment and then placed in a live imaging device (Lumina XR Series III; Perkin-Elmer, Inc., Waltham, MA, USA) to observe fluorescence distribution in vivo at 0.5, 1, 2, 4, 8, 24 and 36 h.

Antitumor effects of PSP-NE and PSP aqueous solution

The cytotoxicities of PSP and PSP-NE were determined by measuring the inhibition of cell growth using an MTT assay (23). The cells were maintained in RPMI 1640 medium with 10% FBS and 0.1 mg/ml penicillin G and 100 U/ml streptomycin in a humidified atmosphere of 5% CO2 at 37°C. The HepG2 cells and MCF-7 cells were seeded separately on a 96-well plate at a cellular density of 5,000 cells/well when at a confluence of ~80%. Following treatment of the cells with the various concentrations of PSP and PSP-NE over 48 h, 20 µl MTT solution was added to the cells. The cell viability was determined at 570 nm in a microplate reader (Synergy HT; BioTek Instruments, Inc., Winooski, VT, USA). Cell toxicity was calculated according to the following equation: Cell viability (%)=Abssample/Abscontrol ×100%, where Abssample is the absorbance of cells in the presence of different formulations, Abscontrol is the absorbance of cells in the absence of drug.

Antioxidant effects of PSPNE and PSP aqueous solution 1 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•) scavenging assay

The DPPH• is a stable free radical, which is often used as a tool to estimate the antioxidant capacity. The DPPH free radical scavenging activities of PSP and PSP-NE were measured using DPPH according to a published method with modifications (7,24). Vitamin C (Vc) was used as a positive control. The sample solution (2 ml), including Vc, PSP or PSP-NE samples at different concentrations (1, 2, 3, 4 and 5 mg/ml) was added to a tube containing 2 ml DPPH solution (0.04 mg/ml in ethanol). Following incubation in the dark for 20 min at 37°C, the absorbance was measured at 517 nm. The DPPH radical scavenging activity (%) was calculated using the following equation (24): Scavenging activity (%)=1−(A1−A2)/A0 ×100%, where A0 is the absorbance of DPPH solution without samples. A1 is the absorbance of DPPH with the samples, and A2 is the absorbance of background solution (distilled water instead of DPPH solution).

Hydroxyl radical (OH•) scavenging assay

The OH• scavenging activities of PSP and PSP-NE were measured following the modified method of Wu et al (24). Vc was also used as a reference material for a positive control. The sample solution (1 ml) with different concentrations (1, 2, 3, 4 and 5 mg/ml), 1 ml FeSO4 solution (9 mmol/l) and 1 ml salicylic acid ethanol solution (9 mmol/l) were fully mixed, and the reaction was started with 1 ml H2O2 solution (8 mmol/l). The absorbance of the mixture solution was measured at 510 nm following incubation at 37°C for 40 min. The OH• scavenging activity was calculated as follows: Scavenging activity (%)=1− (A1−A2)/A0 ×100%, where A0 is the absorbance of the negative control (without samples), A1 is the absorbance of the sample mixture, and A2 is the absorbance of background solution (distilled water instead of H2O2).

Statistical analysis

Quantitative data are expressed as the mean ± standard deviation. Statistical analysis (one-way analysis of variance) was performed with GraphPad Prism. V6.0 software (GraphPad Software, Inc., La Jolla, CA, USA). P<0.05 was considered to indicate a statistically significant difference.

Results

Optimization of the PSP-NE formulation

Surfactant complexes

The seven ratios of surfactant complexes all had good emulsifying ability to form NEs. The results of the pseudo-ternary phase diagram are shown in Fig. 1. The largest NE-forming area was observed at Span 80/Tween-80=3:1, and this ratio was selected as the optimal surfactant complex for subsequent experiments.

Figure 1

Pseudo-ternary phase diagrams of mixed surfactants. (A) Span 80/glyceryl monooleate ratio of 3:2; (B) glyceryl monooleate/Span 80 ratio of 4:3; (C) glycerylmonooleate/Tween-80 ratio of 4:1; (D) Span 80/Tween-80 ratio of 4:1; (E) glyceryl monooleate/Tween-80 ratio of 2:1; (F) Span 80/Tween-80 ratio of 3:1; (G) glyceryl monooleate/Tween-80 ratio of 3:1. Smix, surfactant mix.

Screening of co-surfactant and Km

Among the several commonly used co-surfactants, only anhydrous ethanol formed NE when the Km values were 4:1, 3:1, and 2:1. In addition, it did not significantly change in appearance regardless of storage in either hot or cold temperatures. Therefore, anhydrous ethanol was selected as a co-surfactant. The pseudo-ternary phase diagram drawn by different Km values is shown in Fig. 2. The NE-forming area at c (Km=2:1) was the largest, and was selected for subsequent experiments.

Figure 2

Pseudo-ternary phase diagrams under different K values. (A) K=4:1; (B) K=3:1; (C) K=2:1. K, surfactant and co-surfactant ratio; Smix, surfactant mix.

Oil phase screening

Four oil phases were assessed for their ability to form NE and the results are shown in Fig. 3. The NE-forming areas were as follows: Soybean oil >liquid paraffin >medium-chain fatty acid triglycerides >sesame oil. Therefore, soybean oil was selected as the preferred oil phase for subsequent experiments.

Figure 3

Pseudo-ternary phase diagrams using different oils. (A) Soybean oil; (B) liquid paraffin; (C) medium chain triglyceride; (D) sesame oil. Smix, surfactant mix.

Aqueous phase screening

PSP solution at <20% had no significant effect on NE formation. At higher concentrations, the NE became turbid. As shown in the pseudo-ternary phase diagram (Fig. 4), the NE-forming area was as follows: 1 >2 >5 >10 >20% PSP. There were no significant differences among the areas at 1, 2 and 5% PSP, however, the NE formation was considerably reduced when PSP concentration exceeded 10%. PSP at 10% was the final water phase used in consideration of the drug loading.

Figure 4

Pseudo-ternary phase diagrams using different concentrations of aqueous spirulina polysaccharide solution (A) 1%; (B) 2%; (C) 5%; (D) 10%; (E) 20%. Smix, surfactant mix.

Therefore, the final NE formulation consisted of 11.9% Span 80, 6.0% Tween-80, 9.0% absolute ethanol, 62.8% soybean oil, and 10.3% aqueous PSP.

Characteristics of the PSP-NE formulation

Appearance and type identification

The PSP-NE formed according to the above formulation was light yellow, transparent and uniform. Following high-speed centrifugation, the NE remained in a single phase, and the diffusion rate of the Sudan red exceeded that of the methylene blue (Fig. 5). These observations demonstrated that the PSP-NE was a W/O NE.

Figure 5

Transmission electron microscopy image of the spirulina polysaccharide nanoemulsion (magnification, ×39,000).

Morphological observation and particle size determination

The PSP-NE droplets were homogeneous, spherical and non-adhesive. As shown in Fig. 6, the average particle sizes were 79.93±19 nm, and the PDI values were 0.185±0.04 (n=3).

Figure 6

Particle size and PDI of the spirulina polysaccharide nanoemulsion.

The viscosity and pH values obtained in the determination of empty NE and PSP-NE are shown in Table I.

Table I

pH and viscosity of empty NE and PSP-NE.

Sample	pH	Viscosity(relative to water; °E)
NE only	7.37±0.03	11.68±0.03
PSP-NE	7.24±0.02	12.37±0.06

PSP, spirulina polysaccharide; NE, nanoemulsion.

The PSP-NE did not exhibit a muddy consistency following stratification or sedimentation, and remained clear and transparent. As indicated in Table II, the results of the light experiment showed that PSP-NE was stable for up to 10 days. As indicated in Table III, the PSP-NE was stable for up to 2 months during the accelerated experiment. Together, these observations demonstrated that PSP-NE was stable.

Table II

Light properties of PSP-NE (n=3).

Time (days)	Appearance	Particle size	PSP content (mg/ml)	pH	Viscosity(relative to water; °E)
0	Clear and transparent	79.16±0.76	5.17±0.03	7.23±0.03	11.52±0.04
5	Clear and transparent	79.42±0.65	5.16±0.01	7.21±0.00	11.52±0.05
10	Clear and transparent	80.02±1.06	5.16±0.03	7.18±0.01	11.55±0.05

PSP, spirulina polysaccharide; NE, nanoemulsion.

Table III

Accelerated test results of PSP-NE (n=3).

Time(mins)	Appearance	Particle size	PSP content(mg/ml)	pH	Viscosity(relative to water; °E)
0	Clear and transparent	78.63±0.50	5.16±0.02	7.24±0.01	11.50±0.01
1	Clear and transparent	79.06±0.41	5.15±0.01	7.22±0.02	11.49±0.03
2	Clear and transparent	79.66±0.46	5.12±0.01	7.20±0.02	11.51±0.07
3	Clear, transparent, slight darker color	80.43±1.02	5.10±0.02	7.16±0.02	11.53±0.02
6	Clear, transparent, darker color	80.75±1.30	5.11±0.02	7.14±0.03	11.54±0.03

PSP, spirulina polysaccharide; NE, nanoemulsion.

The content of PSP measured was 5.14±0.06 mg/ml. The entrapment rate measured was 62%.

Biodistribution following oral administration in nude mice

The fluorescence distribution at different time points are shown in Fig. 7A (PSP) and Fig. 7B (PSP-NE). In the PSP group, fluorescence was distributed throughout the body by ~2 h, mostly eliminated by 8 h, and eliminated at 24 h. By contrast, the fluorescence of the PSP-NE group was distributed throughout the body in ~4 h, and the fluorescence intensity was significantly higher than that of the PSP group. In addition, significant fluorescence accumulated in the liver and the kidney, and remained detectable at ~24 h, and almost eliminated at 36 h. These findings indicated that the PSP-NE exhibited a sustained-release and tissue effect.

Figure 7

Distribution of PSP-NE. (A) Fluorescence in nude mice following intragastric administration of PSP and ICG at different time points. PSP, spirulina polysaccharide; NE, nanoemulsion; ICG, indocyanine green. (B) Fluorescence in nude mice following intragastric administration of PSP-ICG-NE at different time points. PSP, spirulina polysaccharide; NE, nanoemulsion; ICG, indocyanine green.

Antitumor assay

As shown in Fig. 8A and B, PSP-NE exhibited a superior inhibitory effect on MCF-7 cells (IC50 at 500 µg/ml) and HepG2 cells (IC50 at 500 µg/ml), compared with PSP (MCF-7 cell IC50 at 1,000 µg/ml; HepG2 cell IC50 at 2,000 µg/ml).

Figure 8

Cytotoxicity of PSP and PSP-NE. (A) In vitro cytotoxicity of PSP and PSP-NE against HepG2 cells. (B) In vitro cytotoxicity of PSP and PSP-NE against MCF-7 cells. PSP, spirulina polysaccharide; NE, nanoemulsion.

Antioxidant assay

In the present study, the potent free radical scavenging ability of PSP and PSP-NE on DPPH• and OH• was determined at concentrations between 1 and 5 mg/ml, which showed concentration-dependent effects. The scavenging abilities increased with increasing concentration. As shown in Fig. 9A and B, Vc exerted higher antioxidant scavenging of DPPH•, but a lower ability to scavenge OH•, compared with PSP or PSP-NE. However, no significant differences were observed in the DPPH• and OH• scavenging activity between PSP-NE and PSP at any concentrations.

Figure 9

(A) Scavenging effect of PSP and PSP-NE on DPPH radicals compared with that of VC. (B) Scavenging activity of PSP and PSP-NE on OH radicals compared with that of VC. PSP, spirulina polysaccharide; NE, nanoemulsion; DPPH, 1 1,1-diphenyl-2-picrylhydrazyl; OH, hydroxyl radical; VC, vitamin C.

Discussion

The surfactant screening process revealed that a single surfactant was not able to form an NE with superior properties. Therefore, using combinations of surfactants is suitable for the adjustment of HLB values and enhancement of emulsion stability. The HLB of Span 80 was 4.3 and that of Tween-80 was 15.0, and a 3:1 mixture ratio produced the desired HLB for soybean oil emulsification.

When the co-surfactant was screened, ethanol was selected, which presented with the largest NE formation area at a Km 2:1. In the process of NE formation, liquid crystal and gel phenomenon (soybean phospholipids as a surfactant) may occur, and alcohol is conducive to the transition of a liquid crystal phase to an NE phase.

When screening the oil phase, the smaller the molecular volume, the higher the ability to dissolve the drug, and the faster the NE formation. Peanut and corn oils formed NE, but the resulting NE appeared turbid at high- or low-temperature storage (data not shown). Other oil phases not only formed NEs with good qualities but also remained unchanged at low and high temperatures. Therefore, liquid paraffin, sesame oil, injection-grade soybean oil, and medium-chain fatty acid triglycerides were selected as alternative oil phases. The screened mixed surfactants and co-surfactants were weighed according to the selected Km value and mixed with the above oil phase, and the resulting formulation was screened, finally confirming that soybean oil was the optimal oil phase.

When the water phase was selected, the effects of PSP concentration on NE formation were observed, and the optimum PSP concentration was determined. The results showed that the PSP concentration within 20% did not markedly affect NE formation, and the NE became turbid at higher PSP concentrations. As polysaccharides are macromolecule materials, the area for NE formation gradually decreased at increased PSP concentrations. On considering the drug loading problem, 10% PSP aqueous solution was finally selected.

The majority of polysaccharides neither absorb light nor chromophores, making them difficult to quantify. However, the present study observed the distribution of the dosage form in the body by using living imaging aids. In this experiment, ICG was embedded in PSP-NE, and the ICG-PSP and ICG-PSP-NE solutions were administered in nude mice in parallel. The body distribution of fluorescence was observed over time. The distribution and elimination rates of the PSP-treated group were faster than those of the PSP-NE group. These results indicated that the prepared NEs exhibited slow release.

The in vitro antitumor experiments on HepG2 and MCF-7 cells demonstrated that PSP-NE had a superior inhibitory effect compared with PSP, which may be attributed to the fact that PSP-NE increased the uptake of cells and increased the effective concentration of drugs.

It has been reported that PSP can affect the metabolism in the body (25). Ravi et al (26) demonstrated that PSP reduced the ratio of low-density lipoprotein to high-density lipoprotein (HDL), decreased the levels of plasma lipid, and modified the total cholesterol and HDL cholesterol levels. PSP has also been found to decrease the levels of postprandial blood glucose and glycosylated hemoglobin (HbA1c), and exhibit long-term glycemic regulation (27). Therefore, further investigation of the glucose and lipid metabolism of PSP-NE is required.

In conclusion, PSP-NE was prepared through a phase transition method, and the surfactant, co-surfactant, oil phase and water phase were screened through the use of a pseudo-ternary phase diagram. The prepared PSP-NE was transparent and uniform. Under TEM, the average particle size was 79.93±19 nm; the PDI was 0.185±0.04, PSP content was 5.14±0.06 mg/ml, and the entrapment rate was 62%. The PSP-NE did not exhibit demulsification in the stability assessment, indicating its stability. The live imaging distributions of PSP and PSP-NE in nude mice were observed with a small-animal live imaging instrument and fluorescein ICG. The distribution and elimination rates of the PSP-treated group were faster than those of the PSP-NE-treated group. Furthermore, the PSP-NE treated group exhibited marked liver and kidney accumulation, indicating that the release of NEs was sustained and specifically targeted to the tissue. The free radical scavenging activity and antitumor ability of PSP-NE were also analyzed. The antioxidant effect of PSP-NE was not affected but it exhibited enhanced antitumor ability. The in vitro experiments showed that PSP-NE had good antioxidant and antitumor abilities.