Literature DB >> 29900273

GC-based chemoprofile of lipophilic compounds in Altaian Ganoderma lucidum sample.

Oleg B Shevelev1, Alisa A Seryapina1, Evgenii L Zavjalov1, Lyudmila A Gerlinskaya1, Tatiana N Goryachkovskaya1, Nikolay M Slynko1, Leonid V Kuibida2, Sergey E Peltek1, Arcady L Markel1,3, Mikhail P Moshkin1,3,4.   

Abstract

The presented data contains information about component composition of lipophilic compounds in Ganoderma lucidum fungal body sample obtained using gas chromatography and subsequent mass spectrometry.

Entities:  

Year:  2018        PMID: 29900273      PMCID: PMC5996614          DOI: 10.1016/j.dib.2018.03.098

Source DB:  PubMed          Journal:  Data Brief        ISSN: 2352-3409


Specifications Table Value of the data The data could be potentially valuable to the scientific community, because it is new data about component composition of Ganoderma lucidum from Altai region. It could be compared to the data of the same fungus from another regions. The data is needed to investigate impact of individual compounds to organism.

Data

See Fig. S1 and Table S1.

Experimental design, materials, and methods

To obtain the fingerprint of the lipophilic compounds of Ganoderma lucidum, 1.0 g of air-dry mass of the crushed fruit body of the fungus was boiled with 10 ml of methyl alcohol for 1 h, the extract was filtered and the filtrate was passed through a column with an Al2O3 layer 15 mm in height for dehydration, 1 ml was taken for analysis by gas chromatography. The analysis was carried out on an Agilent Technologies 6890N chromatograph with a quartz capillary column DB-1 and an Agilent Technologies 5973N quadrupole mass spectrometer. Chromatographic mass spectrometric analysis of the samples was carried out both to measure the total ion current in the scanning regime in the mass range 10–800 amu, and in the mode of selective ion scanning. The identification of components present in the methanolic extract was based on direct comparison of the retention indices and mass spectral data with those for standard compounds, and by computer matching with the Wiley 229, Nist 107, Nist 21 Library, as well as by comparison of the fragmentation patterns of the mass spectra with those reported in the literature [2]. The Kovacs retention indices were calculated from the retention times of Lucero et al. [1].
Subject areaChemistry
More specific subject areaGas chromatography-based chemoprofile of fungal body of Ganoderma lucidum
Type of dataTable and figure
How data was acquiredGas chromatography and mass spectrometry; Agilent Technologies 6890N chromatograph with a quartz capillary column DB-1 and an Agilent Technologies 5973N quadrupole mass spectrometer.
Data formatAnalyzed
Experimental factorsMushrooms were pre-air-dried to constant weight and grinded using a jet mill to a very fine powder, shredded mushrooms were dried in an air thermostat at a temperature of 70 °C to a constant weight.
Experimental featuresTo obtain the fingerprint of the lipophilic compounds of Ganoderma lucidum, 1.0 g of air-dry mass of the crushed fruit body of the fungus was boiled with 10 ml of methyl alcohol for 1 h, the extract was filtered and the filtrate was passed through a column with an Al2O3 layer 15 mm in height for dehydration, 1 ml was taken for analysis by gas chromatography.
Data source locationNovosibirsk, Russia
Data accessibilityData is with this article.
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