Wei Chen1, Wei Zhao2, Sheng Chen3, Li Zhang4, Zhongying Guo5, Lixin Wang1, Jipeng Wang1, Zongren Wan1, Yongqing Hong1, Liang Yu4,6. 1. Department of Respiratory Medicine, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu, China. 2. Department of Pathology, The Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, Jiangsu, China. 3. Department of Pulmonary Surgery, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu, China. 4. Department of Hematology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu, China. 5. Department of Pathology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu, China. 6. Key Laboratory of Hematology, Nanjing Medical University, Nanjing, Jiangsu, China.
Abstract
INTRODUCTION: Non-small cell lung cancer (NSCLC) is the most common cause of cancer-related deaths in the world. MALAT1 and SOX9 have important roles in tumour formation and development in several types of cancers. However, little is known about the function and co-relationship of these 2 factors in NSCLC in vivo. OBJECTIVES: To explore the role of MALAT1 and SOX9 expression relationship, their clinical pathological characteristics and OS on NSCLC patients. METHODS: Paired of primary lung cancer tissues and the matched tumour adjacent tissues were collected in 121 NSCLC patients. MALAT1 and SOX9 mRNA expression was measured by SYBR green q RT-PCR assay. SOX-9 protein expression was measured by streptavidin-peroxidase (SP) staining method. RESULTS: MALAT1and SOX9 expression was higher in NSCLC tissues than the adjacent tissues, and they have positive correlation. Moreover, SOX9 protein expression was higher in NSCLC tissues, especially in MALAT1 mRNA higher expressed NSCLC tissues. MALAT1 and SOX9 mRNA expression were associated with age (x2 =11.474, P = .009), tumour size (x2 =26.839, P = .000), TNM stage (x2 =8.010, P = .046) and LEL. (x2 =53.908, P = .000). NSCLC patients with higher MALAT1 and SOX9 mRNA expression had poorer OS rates. CONCLUSIONS: MALAT1 and SOX9 could be used as prognostic co-biomarker in NSCLC.
INTRODUCTION:Non-small cell lung cancer (NSCLC) is the most common cause of cancer-related deaths in the world. MALAT1 and SOX9 have important roles in tumour formation and development in several types of cancers. However, little is known about the function and co-relationship of these 2 factors in NSCLC in vivo. OBJECTIVES: To explore the role of MALAT1 and SOX9 expression relationship, their clinical pathological characteristics and OS on NSCLCpatients. METHODS: Paired of primary lung cancer tissues and the matched tumour adjacent tissues were collected in 121 NSCLCpatients. MALAT1 and SOX9 mRNA expression was measured by SYBR green q RT-PCR assay. SOX-9 protein expression was measured by streptavidin-peroxidase (SP) staining method. RESULTS: MALAT1and SOX9 expression was higher in NSCLC tissues than the adjacent tissues, and they have positive correlation. Moreover, SOX9 protein expression was higher in NSCLC tissues, especially in MALAT1 mRNA higher expressed NSCLC tissues. MALAT1 and SOX9 mRNA expression were associated with age (x2 =11.474, P = .009), tumour size (x2 =26.839, P = .000), TNM stage (x2 =8.010, P = .046) and LEL. (x2 =53.908, P = .000). NSCLCpatients with higher MALAT1 and SOX9 mRNA expression had poorer OS rates. CONCLUSIONS:MALAT1 and SOX9 could be used as prognostic co-biomarker in NSCLC.