Literature DB >> 2989313

Purification method for the avian adeno-associated virus.

H J Bauer, G Monreal.   

Abstract

Avian adeno-associated virus (AAAV) pre-purified by Uvasol extraction, one discontinuous and two CsCl equilibrium density gradients, was still considerably contaminated with avian adenovirus (CELO strain). Four different approaches were investigated in attempts to improve the elimination of the contaminating CELO virus. The contaminations were assayed by double immunodiffusion and indirect immunofluorescence. The immunoprecipitation of CELO virus with antiserum and protein A-Sepharose is the most effective method of obtaining purified AAAV free of CELO virus.

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Year:  1985        PMID： 2989313     DOI： 10.1016/0166-0934(85)90127-2

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  3 in total

1.  Biological and physicochemical characterization of the major (1.40) and minor (1.45) component of infectious avian adeno-associated virus.

Authors:  H J Bauer; R Schneider; H R Gelderblom; R Lurz; V Friehmelt; G Monreal
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

2.  Avian adeno-associated parvovirus and Marek's disease virus: studies of viral interactions in chicken embryo fibroblasts. Brief report.

Authors:  H J Bauer; G Monreal
Journal:  Arch Virol       Date:  1988       Impact factor: 2.574

3.  Molecular characterization of two strains of the avian adeno-associated virus (AAAV).

Authors:  M Hess; G Paul; S Kling; G Monreal
Journal:  Arch Virol       Date:  1995       Impact factor: 2.574
  3 in total

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