Literature DB >> 2989097

Purification of a new restriction endonuclease, StyI, from Escherichia coli carrying the hsd+ miniplasmid.

K Mise, K Nakajima.   

Abstract

A new restriction endonuclease, StyI, free of contaminating nuclease activities, has been isolated from Escherichia coli carrying the hsd+ miniplasmid of Salmonella typhi origin. In the presence of 10 mM Mg2+, it recognizes and cleaves a hexanucleotide sequence of 5'-C decreases C(AT)(AT)GG. The advantages of the StyI endonuclease include its stability, high yield (more than 2 X 10(3) units/g of wet cells), easy handling of producer cells, and the ability to recognize new sequences, CCAAGG and CCTTGG.

Entities:  

Mesh:

Substances:

Year:  1985        PMID: 2989097     DOI: 10.1016/0378-1119(85)90244-6

Source DB:  PubMed          Journal:  Gene        ISSN: 0378-1119            Impact factor:   3.688


  8 in total

1.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971

2.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1989       Impact factor: 16.971

3.  Cloning, sequencing and expression of the Taq I restriction-modification system.

Authors:  B E Slatko; J S Benner; T Jager-Quinton; L S Moran; T G Simcox; E M Van Cott; G G Wilson
Journal:  Nucleic Acids Res       Date:  1987-12-10       Impact factor: 16.971

4.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1988       Impact factor: 16.971

5.  Occurrence of small Hsd plasmids in Salmonella typhi, Shigella boydii, and Escherichia coli.

Authors:  Y Yoshida; K Mise
Journal:  J Bacteriol       Date:  1986-02       Impact factor: 3.490

6.  Widespread occurrence of specific restriction endonucleases in Salmonella infantis, Salmonella thompson, and Salmonella blockley isolated from humans in Japan.

Authors:  M Miyahara; Y Kudoh; K Mise
Journal:  Appl Environ Microbiol       Date:  1990-07       Impact factor: 4.792

Review 7.  Restriction endonucleases: classification, properties, and applications.

Authors:  Raymond J Williams
Journal:  Mol Biotechnol       Date:  2003-03       Impact factor: 2.860

8.  An alternative approach to study the enzymatic specificities of the CfrBI restriction-modification system.

Authors:  Marina V Zakharova; Irina V Beletskaya; Elena M Ibryashkina; Alexander S Solonin
Journal:  Heliyon       Date:  2019-06-08
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.