| Literature DB >> 2989097 |
Abstract
A new restriction endonuclease, StyI, free of contaminating nuclease activities, has been isolated from Escherichia coli carrying the hsd+ miniplasmid of Salmonella typhi origin. In the presence of 10 mM Mg2+, it recognizes and cleaves a hexanucleotide sequence of 5'-C decreases C(AT)(AT)GG. The advantages of the StyI endonuclease include its stability, high yield (more than 2 X 10(3) units/g of wet cells), easy handling of producer cells, and the ability to recognize new sequences, CCAAGG and CCTTGG.Entities:
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Year: 1985 PMID: 2989097 DOI: 10.1016/0378-1119(85)90244-6
Source DB: PubMed Journal: Gene ISSN: 0378-1119 Impact factor: 3.688