Se Jin Lee1, Yi-Ting Yang1, Sihyeon Kim1, Mi Rong Lee1, Jong Cheol Kim1, So Eun Park1, Muktadir S Hossain1, Tae Young Shin1, Yu-Shin Nai2, Jae Su Kim1,3. 1. Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, South Korea. 2. Department of Biotechnology and Animal Science, National Ilan University, Ilan, Taiwan. 3. Plant Medical Research Center, College of Agricultural and Life Sciences, Chonbuk National University, Jenoju, South Korea.
Abstract
BACKGROUND: Entomopathogenic Beauveria bassiana has been used as a biocontrol agent for insect pests, but its effect at the molecular level on the hosts has not been studied in detail. Herein, we performed transcriptome analysis of bean bug, Riptortus pedestris (Hemiptera: Alydidae) in response to infection with a highly virulent strain of B. bassiana JEF-007 (Bb JEF-007). RESULTS: Based on RNA-seq data from R. pedestris infected with Bb JEF-007 compared with non-infected bean bugs, infection was assumed to strongly activate (i) the energy production pathway by expressing dehydrogenases, (ii) metabolic pathways by expressing secreted proteins, GTPase, MBF2 transcription factor family, pigment-dispersing factor, antioxidants, and cuticle proteins, and (iii) the immune response pathway by expressing serine-threonine kinase in Toll pathway of bean bug. CONCLUSION: We have established the platform for functional studies of the genes required for an immune response against entomopathogenic fungi like B. bassiana in the bean bug, R. pedestris. Moreover, this study also paves the way for genetic modification of B. bassiana to combat with the defense mechanism of R. pedestris.
BACKGROUND: Entomopathogenic Beauveria bassiana has been used as a biocontrol agent for insect pests, but its effect at the molecular level on the hosts has not been studied in detail. Herein, we performed transcriptome analysis of bean bug, Riptortus pedestris (Hemiptera: Alydidae) in response to infection with a highly virulent strain of B. bassiana JEF-007 (Bb JEF-007). RESULTS: Based on RNA-seq data from R. pedestris infected with Bb JEF-007 compared with non-infected bean bugs, infection was assumed to strongly activate (i) the energy production pathway by expressing dehydrogenases, (ii) metabolic pathways by expressing secreted proteins, GTPase, MBF2 transcription factor family, pigment-dispersing factor, antioxidants, and cuticle proteins, and (iii) the immune response pathway by expressing serine-threonine kinase in Toll pathway of bean bug. CONCLUSION: We have established the platform for functional studies of the genes required for an immune response against entomopathogenic fungi like B. bassiana in the bean bug, R. pedestris. Moreover, this study also paves the way for genetic modification of B. bassiana to combat with the defense mechanism of R. pedestris.
Authors: Laila Gasmi; Sehyeon Baek; Jong Cheol Kim; Sihyeon Kim; Mi Rong Lee; So Eun Park; Tae Young Shin; Se Jin Lee; Bruce L Parker; Jae Su Kim Journal: Sci Rep Date: 2021-01-08 Impact factor: 4.379
Authors: Sihyeon Kim; Jong Cheol Kim; Se Jin Lee; Mi Rong Lee; So Eun Park; Dongwei Li; Sehyeon Baek; Tae Young Shin; Jae Su Kim Journal: BMC Genomics Date: 2020-11-27 Impact factor: 3.969