Diego Pomarè Montin1, Ghada Ankawi1,2, Anna Lorenzin1,3, Mauro Neri1,3, Carlotta Caprara1,4, Claudio Ronco1,3. 1. IRRIV-International Renal Research Institute Vicenza, Vicenza, Italy. 2. Department of Internal Medicine and Nephrology, King Abdulaziz University Hospital, Jeddah, Saudi Arabia. 3. Department of Nephrology, Dialysis and Transplant, San Bortolo Hospital, Vicenza, Italy. 4. Istituto di Ricerca Pediatrica, Città della Speranza, Laboratorio di Genetica Clinica ed Epidemiologica, Padova, Italy.
Abstract
BACKGROUND/AIMS: The use of adsorption cartridges for hemoperfusion (HP) is rapidly evolving. For these devices, the potential induced cytotoxicity is an important issue. The aim of this study was to investigate potential in vitro cytotoxic effects of different sorbent cartridges, HA130, HA230, HA330, HA380 (Jafron, China), on U937 monocytes. METHODS: Monocytes were exposed to the sorbent material in static and dynamic manners. In static test, cell medium samples were collected after 24 h of incubation in the cartridges. In dynamic test, HP modality has been carried out and samples at 30, 60, 90, and 120 min were collected. RESULTS: Compared to control samples, there was no evidence of increased necrosis or apoptosis in monocytes exposed to the cartridges both in the static and dynamic tests. CONCLUSION: Our in vitro testing suggests that HA cartridges carry an optimal level of biocompatibility and their use in HP is not associated with adverse reactions or signs of cytotoxicity.
BACKGROUND/AIMS: The use of adsorption cartridges for hemoperfusion (HP) is rapidly evolving. For these devices, the potential induced cytotoxicity is an important issue. The aim of this study was to investigate potential in vitro cytotoxic effects of different sorbent cartridges, HA130, HA230, HA330, HA380 (Jafron, China), on U937 monocytes. METHODS: Monocytes were exposed to the sorbent material in static and dynamic manners. In static test, cell medium samples were collected after 24 h of incubation in the cartridges. In dynamic test, HP modality has been carried out and samples at 30, 60, 90, and 120 min were collected. RESULTS: Compared to control samples, there was no evidence of increased necrosis or apoptosis in monocytes exposed to the cartridges both in the static and dynamic tests. CONCLUSION: Our in vitro testing suggests that HA cartridges carry an optimal level of biocompatibility and their use in HP is not associated with adverse reactions or signs of cytotoxicity.
Authors: Federico Nalesso; Federica L Stefanelli; Valentina Di Vico; Leda Cattarin; Irene Cirella; Giuseppe Scaparrotta; Francesco Garzotto; Lorenzo A Calò Journal: Biomedicines Date: 2022-08-19
Authors: Alan Saúl Álvarez-Suárez; Syed G Dastager; Nina Bogdanchikova; Daniel Grande; Alexey Pestryakov; Juan Carlos García-Ramos; Graciela Lizeth Pérez-González; Karla Juárez-Moreno; Yanis Toledano-Magaña; Elena Smolentseva; Juan Antonio Paz-González; Tatiana Popova; Lyubov Rachkovskaya; Vadim Nimaev; Anastasia Kotlyarova; Maksim Korolev; Andrey Letyagin; Luis Jesús Villarreal-Gómez Journal: Micromachines (Basel) Date: 2020-04-22 Impact factor: 2.891