Huiying Zhang1, Zhigang Song2, Huiju Yu3, Xiaoling Zhang4, Shanshan Xu5, Zhong Li6, Jingzhi Li7, Hongke Xu8, Zhenghong Yuan9, Hongwei Ma10, Zhigang Yi11, Yunwen Hu12. 1. Department of pathogen diagnosis and biosafety, Shanghai public health clinical center, Fudan University, Shanghai, 201508, PR China. Electronic address: 15111300003@fudan.edu.cn. 2. Department of pathogen diagnosis and biosafety, Shanghai public health clinical center, Fudan University, Shanghai, 201508, PR China. Electronic address: ys02261059@163.com. 3. Department of pediatrics infectious disease, Xinhua hospital affiliated to Shanghai Jiao Tong University School of medicine, Shanghai, 201508, PR China. Electronic address: yhj3479@163.com. 4. Department of pathogen diagnosis and biosafety, Shanghai public health clinical center, Fudan University, Shanghai, 201508, PR China. Electronic address: missann111@126.com. 5. Department of pediatrics infectious disease, Xinhua hospital affiliated to Shanghai Jiao Tong University School of medicine, Shanghai, 201508, PR China. Electronic address: xushanshan198410@163.com. 6. Nano-Bio-Med department, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, PR China. Electronic address: chenxudao@163.com. 7. Nano-Bio-Med department, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, PR China. Electronic address: jzli2013@sinano.ac.cn. 8. Nano-Bio-Med department, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, PR China. Electronic address: hkxu2012@sinano.ac.cn. 9. Key Laboratory of medical molecular virology and department of medical microbiology, School of basic medical sciences, Shanghai medical college of Fudan University, Shanghai, 20003, PR China. Electronic address: yuanzhenghong@shphc.org.cn. 10. Nano-Bio-Med department, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, PR China. Electronic address: hwma2008@sinano.ac.cn. 11. Department of pathogen diagnosis and biosafety, Shanghai public health clinical center, Fudan University, Shanghai, 201508, PR China; Key Laboratory of medical molecular virology and department of medical microbiology, School of basic medical sciences, Shanghai medical college of Fudan University, Shanghai, 20003, PR China. Electronic address: zgyi@fudan.edu.cn. 12. Department of pathogen diagnosis and biosafety, Shanghai public health clinical center, Fudan University, Shanghai, 201508, PR China. Electronic address: ywhu2017@163.com.
Abstract
BACKGROUND: Enteroviruses cause hand, foot and mouth disease (HFMD). The host B-cells recognize the viral proteins and provoke humoral responses. Deciphering the B-cell responses to the viral epitopes helps diagnosis and vaccine development. OBJECTIVES: The objective of the present study was to investigate for the first time the landscape of genome-wide linear B-cell epitopes of enterovirus 71 in HFMD population. STUDY DESIGN: The peptides encompassing the entire coding region of EV71 were chemically synthesized and displayed on a microarray. The peptide microarray was used to screen serum samples from an HFMD population, including EV71-, CAV10-, CAV16- and CAV6-infected patients. We identified the dominant epitope-containing-peptides (DECPs) that react with the sera of more than 20% of the HFMD population and the common DECPs that cross-react with the sera from other enteroviruses-infected population. RESULTS: Ten DECPs reacting with IgM and 9 DECPs reacting with IgG antibodies were identified, of which, 6 IgM and 5 IgG common DECPs cross-reacted with the sera from other enteroviruses. Some DECPs preferentially reacted with IgG or IgM antibodies and some epitope-antibody interactions correlated with the severity of HFMD. CONCLUSIONS: We uncovered the DECPs and the common DECPs among a group of enteroviruses in HFMD population and found that some epitope-antibody reactions were associated with the outcome of HFMD. These data may guide developing vaccines against the enteroviruses and help the diagnosis and prognosis of HFMD.
BACKGROUND: Enteroviruses cause hand, foot and mouth disease (HFMD). The host B-cells recognize the viral proteins and provoke humoral responses. Deciphering the B-cell responses to the viral epitopes helps diagnosis and vaccine development. OBJECTIVES: The objective of the present study was to investigate for the first time the landscape of genome-wide linear B-cell epitopes of enterovirus 71 in HFMD population. STUDY DESIGN: The peptides encompassing the entire coding region of EV71 were chemically synthesized and displayed on a microarray. The peptide microarray was used to screen serum samples from an HFMD population, including EV71-, CAV10-, CAV16- and CAV6-infectedpatients. We identified the dominant epitope-containing-peptides (DECPs) that react with the sera of more than 20% of the HFMD population and the common DECPs that cross-react with the sera from other enteroviruses-infected population. RESULTS: Ten DECPs reacting with IgM and 9 DECPs reacting with IgG antibodies were identified, of which, 6 IgM and 5 IgG common DECPs cross-reacted with the sera from other enteroviruses. Some DECPs preferentially reacted with IgG or IgM antibodies and some epitope-antibody interactions correlated with the severity of HFMD. CONCLUSIONS: We uncovered the DECPs and the common DECPs among a group of enteroviruses in HFMD population and found that some epitope-antibody reactions were associated with the outcome of HFMD. These data may guide developing vaccines against the enteroviruses and help the diagnosis and prognosis of HFMD.
Authors: Niila V V Saarinen; Virginia M Stone; Minna M Hankaniemi; Magdalena A Mazur; Tytti Vuorinen; Malin Flodström-Tullberg; Heikki Hyöty; Vesa P Hytönen; Olli H Laitinen Journal: Viruses Date: 2020-01-09 Impact factor: 5.048
Authors: Amy B Rosenfeld; Edmund Qian Long Shen; Michaela Melendez; Nischay Mishra; W Ian Lipkin; Vincent R Racaniello Journal: mBio Date: 2022-01-18 Impact factor: 7.786