Doo Sun Sim1, Weon Kim2, Kyung Hye Lee3, Ho Chun Song4, Ja Hye Kim4, Dae Sung Park1, Kyung Seob Lim5, Jong Shin Woo3, Young Joon Hong1, Youngkeun Ahn1, Hyun Sook Hong6, Youngsook Son6, Myung Ho Jeong7. 1. The Heart Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health, Welfare and Family Affairs, Gwangju, Republic of Korea. 2. Department of Cardiovascular Medicine, Kyung Hee University, Seoul, Republic of Korea. Electronic address: mylovekw@hanmail.net. 3. Department of Cardiovascular Medicine, Kyung Hee University, Seoul, Republic of Korea. 4. Department of Nuclear Medicine, Chonnam National University Hospital, Gwangju, Republic of Korea. 5. National Primate Research Center & Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, Chungbuk, Republic of Korea. 6. College of Medicine, , Kyung Hee Institute for Regenerative Medicine, Kyung Hee University, Seoul, Republic of Korea. 7. The Heart Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health, Welfare and Family Affairs, Gwangju, Republic of Korea. Electronic address: myungho@chollian.net.
Abstract
BACKGROUND: Substance P (SP) may attenuate ischemia-reperfusion injury by reducing inflammation. We assessed cardioprotective effect of SP in a porcine model of acute myocardial infarction (AMI). METHODS: AMI was induced by occlusion of the left anterior descending artery on 28 swine, randomized to SP 5 nmol/kg (group 1, n = 14) and normal saline (group 2, n = 14) given intravenously 5 min before reperfusion. Blood samples were collected at baseline, 3 days and 4 weeks. Echocardiography and myocardial perfusion single photon emission computed tomography (SPECT) were performed at 1 week and 4 weeks. Histomorphometric infarct size assessment was done at 4 weeks. RESULTS: Left ventricular (LV) ejection fraction (EF) (LVEF) after AMI induction was higher in group 1 than group 2 (37.9 ± 4.6% vs. 29.4 ± 3.2%, p = 0.001) but not different at 4 weeks. No significant difference was observed in perfusion defect extent and total perfusion defect on SPECT at 1 week and 4 weeks. Pathologic infarct size (% LV) was significantly smaller in group 1 than group 2 (2.4 ± 2.3% vs. 5.7 ± 2.5%, p = 0.020). The ratio of neutrophil to lymphocyte on day 3 and serum creatinine concentration at 4 weeks after AMI were lower in group 1. CONCLUSIONS: In a porcine model of AMI, SP improved LVEF early post-MI and reduced infarct size. SP may be beneficial in reducing inflammation and ischemia-reperfusion injury after AMI.
BACKGROUND: Substance P (SP) may attenuate ischemia-reperfusion injury by reducing inflammation. We assessed cardioprotective effect of SP in a porcine model of acute myocardial infarction (AMI). METHODS: AMI was induced by occlusion of the left anterior descending artery on 28 swine, randomized to SP 5 nmol/kg (group 1, n = 14) and normal saline (group 2, n = 14) given intravenously 5 min before reperfusion. Blood samples were collected at baseline, 3 days and 4 weeks. Echocardiography and myocardial perfusion single photon emission computed tomography (SPECT) were performed at 1 week and 4 weeks. Histomorphometric infarct size assessment was done at 4 weeks. RESULTS: Left ventricular (LV) ejection fraction (EF) (LVEF) after AMI induction was higher in group 1 than group 2 (37.9 ± 4.6% vs. 29.4 ± 3.2%, p = 0.001) but not different at 4 weeks. No significant difference was observed in perfusion defect extent and total perfusion defect on SPECT at 1 week and 4 weeks. Pathologic infarct size (% LV) was significantly smaller in group 1 than group 2 (2.4 ± 2.3% vs. 5.7 ± 2.5%, p = 0.020). The ratio of neutrophil to lymphocyte on day 3 and serum creatinine concentration at 4 weeks after AMI were lower in group 1. CONCLUSIONS: In a porcine model of AMI, SP improved LVEF early post-MI and reduced infarct size. SP may be beneficial in reducing inflammation and ischemia-reperfusion injury after AMI.
Authors: Alexander Widiapradja; Ainsley O Kasparian; Samuel L McCaffrey; Lauren L Kolb; John D Imig; Jessica L Lacey; Giselle C Melendez; Scott P Levick Journal: Cells Date: 2021-10-05 Impact factor: 6.600