Sameer Ahmed Alsharapy1,2, Betitera Yanat3, Lorena Lopez-Cerero4,5,6, Samar Saeed Nasher2, Paula Díaz-De-Alba4, Álvaro Pascual4,5,6,7, José-Manuel Rodríguez-Martínez5,6,7, Abdelaziz Touati1. 1. Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia, Bejaia, Algeria. 2. Department of Microbiology, National Center of Public Health Laboratories (NCPHL), Sanaa, Yemen. 3. Département de Microbiologie, FSNV, Université de Bejaia, Bejaia, Algeria. 4. Unidad de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Universitario Virgen Macarena, Seville, Spain. 5. Departamento de Microbiología, Universidad de Sevilla, Seville, Spain. 6. Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain. 7. Red Española de Investigación en Patología Infecciosa (REIPI), Instituto de Salud Carlos III, Madrid, Spain.
Abstract
Objective: The aim of this study was to characterize the O25b/ST131 clone in ciprofloxacin-resistant Escherichia coli isolates from Yemen. Materials and Methods: A total of 41 ciprofloxacin-resistant E. coli strains were collected from clinical samples of inpatients and outpatients from Sana'a (Yemen) from January to December 2013. Antimicrobial susceptibility testing, polymerase chain reaction amplification, and sequencing were used for detection of plasmid-mediated quinolone resistance determinants, extended-spectrum beta-lactamases genes and mutations in the quinolone resistance-determining regions of the target genes gyrA and parC. Genetic relatedness of E. coli isolates was determined by pulsed-field gel electrophoresis (PFGE). O25b/ST131 clone detection was performed using polymerase chain reaction of O25b rfb and allele 3 of the pabB gene and by a multilocus sequence typing. Results: All E. coli isolates contained the aac(6')Ib-cr gene associated with blaCTX-M-15 and qnrS genes in 63.4% and 12.2%, respectively. A rate of 36.6% (15/41) of O25b/ST131 E. coli isolates were identified belonging to the H30-Rx subclone producing both CTX-M-15 and Aac(6')Ib-cr enzymes and carrying two substitutions in GyrA (Ser83Leu/Asp87Asn) and two substitutions in ParC (Ser80Ile/Glu84Val). Most of them were uropathogenic unrelated E. coli isolates recovered from outpatients. Conclusion: This is the first report of a high prevalence of E. coli O25b/ST131 from Yemen.
Objective: The aim of this study was to characterize the O25b/ST131 clone in ciprofloxacin-resistant Escherichia coli isolates from Yemen. Materials and Methods: A total of 41 ciprofloxacin-resistant E. coli strains were collected from clinical samples of inpatients and outpatients from Sana'a (Yemen) from January to December 2013. Antimicrobial susceptibility testing, polymerase chain reaction amplification, and sequencing were used for detection of plasmid-mediated quinolone resistance determinants, extended-spectrum beta-lactamases genes and mutations in the quinolone resistance-determining regions of the target genes gyrA and parC. Genetic relatedness of E. coli isolates was determined by pulsed-field gel electrophoresis (PFGE). O25b/ST131 clone detection was performed using polymerase chain reaction of O25b rfb and allele 3 of the pabB gene and by a multilocus sequence typing. Results: All E. coli isolates contained the aac(6')Ib-cr gene associated with blaCTX-M-15 and qnrS genes in 63.4% and 12.2%, respectively. A rate of 36.6% (15/41) of O25b/ST131E. coli isolates were identified belonging to the H30-Rx subclone producing both CTX-M-15 and Aac(6')Ib-cr enzymes and carrying two substitutions in GyrA (Ser83Leu/Asp87Asn) and two substitutions in ParC (Ser80Ile/Glu84Val). Most of them were uropathogenic unrelated E. coli isolates recovered from outpatients. Conclusion: This is the first report of a high prevalence of E. coli O25b/ST131 from Yemen.
Entities:
Keywords:
Aac(6′)Ib-cr; CTX-M-15; E. coli,; O25b-ST131; Yemen
Authors: Minh-Duy Phan; Kate M Peters; Laura Alvarez Fraga; Steven C Wallis; Steven J Hancock; Nguyen Thi Khanh Nhu; Brian M Forde; Michelle J Bauer; David L Paterson; Scott A Beatson; Jeffrey Lipman; Mark A Schembri Journal: Antimicrob Agents Chemother Date: 2021-11-15 Impact factor: 5.938
Authors: Dmitriy Li; Ethan R Wyrsch; Paarthiphan Elankumaran; Monika Dolejska; Marc S Marenda; Glenn F Browning; Rhys N Bushell; Jessica McKinnon; Piklu Roy Chowdhury; Nola Hitchick; Natalie Miller; Erica Donner; Barbara Drigo; Dave Baker; Ian G Charles; Timothy Kudinha; Veronica M Jarocki; Steven Philip Djordjevic Journal: Microb Genom Date: 2021-12