| Literature DB >> 2987993 |
R Gardner, J McAnulty, E Feher, D Lane.
Abstract
Miniplasmids derived by deletion of DNA from the F plasmid secondary replicon have been tested for the ability to replicate and to express incompatibility with the IncFI plasmid, ColV3-K30. The results demonstrate that the minimal rep region of the secondary replicon lies within a 1.9-kb sequence (33.7F-35.6F kb), and that an inc region, presumably involved in replication control, is present in a 0.45-kb portion of the rep region (33.7F-34.15F kb). In addition, the secondary replicon was found not to require DNA polymerase I activity.Entities:
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Year: 1985 PMID: 2987993 DOI: 10.1016/0147-619x(85)90067-8
Source DB: PubMed Journal: Plasmid ISSN: 0147-619X Impact factor: 3.466