Literature DB >> 2987924

Generation of a dominant 8-MDa deletion at the left terminus of vaccinia virus DNA.

E Paez, S Dallo, M Esteban.   

Abstract

Vaccinia virus mutants were obtained in high frequency from mouse Friend erythroleukemia (FEL) cells persistently infected with this virus, which contains a large (122-MDa) DNA. During long-term cell passages viral particles with deletions of the DNA are generated in FEL cells. These mutants have a major 8-MDa deletion starting between 2.2 and 3.2 MDa from the left terminus of the viral genome. More than half of the left end terminal repetition is deleted. These mutants have reduced infectivity compared to wild-type virus. The ease with which vaccinia virus mutants are obtained in FEL cells should provide a suitable system for generating mutants with other poxvirus and permit study of the genetic basis of virulence for this group of viruses.

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Year:  1985        PMID： 2987924      PMCID： PMC397776          DOI： 10.1073/pnas.82.10.3365

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

1.  HindIII and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA.

Authors:  R Wittek; A Menna; D Schümperli; S Stoffel; H K Müller; R Wyler
Journal:  J Virol       Date:  1977-09       Impact factor: 5.103

2.  Spontaneous deletions and duplications of sequences in the genome of cowpox virus.

Authors:  D J Pickup; B S Ink; B L Parsons; W Hu; W K Joklik
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

3.  The white pock (mu) mutants of rabbit poxvirus. III. Terminal DNA sequence duplication and transposition in rabbit poxvirus.

Authors:  R W Moyer; R L Graves; C T Rothe
Journal:  Cell       Date:  1980-11       Impact factor: 41.582

4.  Intragenomic sequence transposition in monkeypox virus.

Authors:  J J Esposito; C D Cabradilla; J H Nakano; J F Obijeski
Journal:  Virology       Date:  1981-03       Impact factor: 3.616

5.  Tandem repeats within the inverted terminal repetition of vaccinia virus DNA.

Authors:  R Wittek; B Moss
Journal:  Cell       Date:  1980-08       Impact factor: 41.582

6.  Hybridization selection and cell-free translation of mRNA's encoded within the inverted terminal repetition of the vaccinia virus genome.

Authors:  J A Cooper; R Wittek; B Moss
Journal:  J Virol       Date:  1981-01       Impact factor: 5.103

7.  The white pock mutants of rabbit poxvirus. I. Spontaneous host range mutants contain deletions.

Authors:  R W Moyer; C T Rothe
Journal:  Virology       Date:  1980-04-15       Impact factor: 3.616

8.  Inverted terminal repetition in vaccinia virus DNA encodes early mRNAs.

Authors:  R Wittek; E Barbosa; J A Cooper; C F Garon; H Chan; B Moss
Journal:  Nature       Date:  1980-05-01       Impact factor: 49.962

9.  Restriction endonuclease analysis of red cowpox virus and its white pock variant.

Authors:  L C Archard; M Mackett
Journal:  J Gen Virol       Date:  1979-10       Impact factor: 3.891

10.  Biogenesis of poxviruses: mirror-image deletions in vaccinia virus DNA.

Authors:  G McFadden; S Dales
Journal:  Cell       Date:  1979-09       Impact factor: 41.582
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  17 in total

1.  Antibodies directed against a synthetic peptide enable detection of a protein encoded by a vaccinia virus host range gene that is conserved within the Orthopoxvirus genus.

Authors:  S Gillard; D Spehner; R Drillien; A Kirn
Journal:  J Virol       Date:  1989-04       Impact factor: 5.103

2.  Mapping and nucleotide sequence of the vaccinia virus gene that encodes a 14-kilodalton fusion protein.

Authors:  J F Rodriguez; M Esteban
Journal:  J Virol       Date:  1987-11       Impact factor: 5.103

3.  Plaque size phenotype as a selectable marker to generate vaccinia virus recombinants.

Authors:  J F Rodriguez; M Esteban
Journal:  J Virol       Date:  1989-02       Impact factor: 5.103

4.  Highly attenuated vaccinia virus mutants for the generation of safe recombinant viruses.

Authors:  D Rodriguez; J R Rodriguez; J F Rodriguez; D Trauber; M Esteban
Journal:  Proc Natl Acad Sci U S A       Date:  1989-02       Impact factor: 11.205

5.  Virus attenuation and identification of structural proteins of vaccinia virus that are selectively modified during virus persistence.

Authors:  E Paez; S Dallo; M Esteban
Journal:  J Virol       Date:  1987-08       Impact factor: 5.103

6.  A 14,000-Mr envelope protein of vaccinia virus is involved in cell fusion and forms covalently linked trimers.

Authors:  J F Rodriguez; E Paez; M Esteban
Journal:  J Virol       Date:  1987-02       Impact factor: 5.103

7.  Establishment of a Vero cell line persistently infected with African swine fever virus.

Authors:  J Salas; E Viñuela
Journal:  J Virol       Date:  1986-05       Impact factor: 5.103

8.  Structural and functional studies of a 39,000-Mr immunodominant protein of vaccinia virus.

Authors:  J S Maa; M Esteban
Journal:  J Virol       Date:  1987-12       Impact factor: 5.103

9.  Regulated expression of nuclear genes by T3 RNA polymerase and lac repressor, using recombinant vaccinia virus vectors.

Authors:  D Rodriguez; Y W Zhou; J R Rodriguez; R K Durbin; V Jimenez; W T McAllister; M Esteban
Journal:  J Virol       Date:  1990-10       Impact factor: 5.103

10.  Attenuated and replication-competent vaccinia virus strains M65 and M101 with distinct biology and immunogenicity as potential vaccine candidates against pathogens.

Authors:  Lucas Sánchez-Sampedro; Carmen Elena Gómez; Ernesto Mejías-Pérez; Eva Pérez-Jiménez; Juan Carlos Oliveros; Mariano Esteban
Journal:  J Virol       Date:  2013-04-17       Impact factor: 5.103
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