Literature DB >> 2987793

Characterization of a factor that can prevent random transcription of cloned rDNA and its probable relationship to poly(ADP-ribose) polymerase.

R N Kurl, S T Jacob.   

Abstract

A factor which eliminated nonspecific transcription of cloned rat rDNA was extensively purified from rat mammary adenocarcinoma ascites cells by successive fractionations on DEAE-Sephadex and heparin-Sepharose columns. The fractions containing RNA polymerase I (HS-B) and fractions eluting thereafter (HS-C) from the heparin-Sepharose column were pooled separately. Addition of HS-C to HS-B prevented random transcription of rDNA and yielded an accurate rDNA transcript with negligible non-specific transcription. The factor was essentially homogeneous and corresponded to Poly(ADP-ribose) polymerase with respect to molecular weight, dependence on DNA for its activity and its ability to undergo auto ADP-ribosylation. The total amount of protein in the transcription assay was approximately 2 micrograms, which indicates a high degree of purity of all the factors required for specific transcription of rDNA.

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Year:  1985        PMID： 2987793      PMCID： PMC340976          DOI： 10.1093/nar/13.1.89

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  40 in total

1.  Studies on the control of ribosomal RNA synthesis in HeLa cells.

Authors:  C J Chesterton; B E Coupar; P H Butterworth; M H Green
Journal:  Eur J Biochem       Date:  1975-09-01

2.  RNA polymerases from a rat hepatoma. Partial purification and comparison of properties with corresponding liver enzymes.

Authors:  K M Rose; P A Ruch; H P Morris; S T Jacob
Journal:  Biochim Biophys Acta       Date:  1976-04-15

3.  Evidence for an extranucleolar mechanism of actinomycin D action.

Authors:  T J Lindell
Journal:  Nature       Date:  1976-09-23       Impact factor: 49.962

4.  Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors:  A J Berk; P A Sharp
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

5.  Alterations in DNA-dependent RNA polymerase I and II from rat liver by thioacetamide: preferential increase in the level of chromatin-associated nucleolar RNA polymerase IB.

Authors:  T B Leonard; S T Jacob
Journal:  Biochemistry       Date:  1977-10-04       Impact factor: 3.162

6.  Resistance of hepatic RNA polymerases to compounds effecting RNA and protein synthesis in vivo.

Authors:  B J Benecke; A Ferencz; K H Seifart
Journal:  FEBS Lett       Date:  1973-04-01       Impact factor: 4.124

7.  Mechanism of early effect of hydrocortisone on the transcriptional process: stimulation of the activities of purified rat liver nucleolar RNA polymerases.

Authors:  E M Sajdel; S T Jacob
Journal:  Biochem Biophys Res Commun       Date:  1971-11-05       Impact factor: 3.575

8.  Evidence for extranucleolar control of RNA synthesis in the nucleolus.

Authors:  S T Jacob; W Muecke; E M Sajdel; H N Munro
Journal:  Biochem Biophys Res Commun       Date:  1970-07-27       Impact factor: 3.575

9.  The absence of a human-specific ribosomal DNA transcription factor leads to nucleolar dominance in mouse greater than human hybrid cells.

Authors:  R Miesfeld; B Sollner-Webb; C Croce; N Arnheim
Journal:  Mol Cell Biol       Date:  1984-07       Impact factor: 4.272

10.  The rapid turnover of RNA polymerase of rat liver nucleolus, and of its messenger RNA.

Authors:  F L Yu; P Feigelson
Journal:  Proc Natl Acad Sci U S A       Date:  1972-10       Impact factor: 11.205
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  11 in total

Review 1.  Poly(ADP-ribosyl)ation reactions in the regulation of nuclear functions.

Authors:  D D'Amours; S Desnoyers; I D'Silva; G G Poirier
Journal:  Biochem J       Date:  1999-09-01       Impact factor: 3.857

Review 2.  Transcription of eukaryotic ribosomal RNA gene.

Authors:  S T Jacob
Journal:  Mol Cell Biochem       Date:  1986-04       Impact factor: 3.396

3.  Isolation, fractionation and reconstitution of a nuclear extract capable of transcribing ribosomal DNA.

Authors:  R E Haglund; L I Rothblum
Journal:  Mol Cell Biochem       Date:  1987-01       Impact factor: 3.396

4.  Factors and nucleotide sequences that direct ribosomal DNA transcription and their relationship to the stable transcription complex.

Authors:  J Tower; V C Culotta; B Sollner-Webb
Journal:  Mol Cell Biol       Date:  1986-10       Impact factor: 4.272

5.  Purification and characterization of a DNA-binding heterodimer of 52 and 100 kDa from HeLa cells.

Authors:  W W Zhang; L X Zhang; R K Busch; J Farrés; H Busch
Journal:  Biochem J       Date:  1993-02-15       Impact factor: 3.857

Review 6.  Molecular and biochemical features of poly (ADP-ribose) metabolism.

Authors:  D Lautier; J Lagueux; J Thibodeau; L Ménard; G G Poirier
Journal:  Mol Cell Biochem       Date:  1993-05-26       Impact factor: 3.396

7.  ADP-ribosylation and gene expression.

Authors:  P Zahradka; L Yau
Journal:  Mol Cell Biochem       Date:  1994-09       Impact factor: 3.396

8.  Role of DNA topoisomerase I in the transcription of supercoiled rRNA gene.

Authors:  L C Garg; S DiAngelo; S T Jacob
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

9.  Characterization of factors that direct transcription of rat ribosomal DNA.

Authors:  S D Smith; E Oriahi; D Lowe; H F Yang-Yen; D O'Mahony; K Rose; K Chen; L I Rothblum
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

10.  Inhibition of calf thymus type II DNA topoisomerase by poly(ADP-ribosylation).

Authors:  M K Darby; B Schmitt; J Jongstra-Bilen; H P Vosberg
Journal:  EMBO J       Date:  1985-08       Impact factor: 11.598
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