| Literature DB >> 29876808 |
Xuefeng Peng1, Candice L Swift1, Michael K Theodorou2, Michelle A O'Malley3.
Abstract
The rapid development of molecular biology and bioinformatics has fueled renewed interests in anaerobic fungi from the phylum Neocallimastigomycota. This chapter presents well-established methods for isolation, routine cultivation, and cryopreservation of anaerobic fungi. Moreover, detailed nucleic acid extraction protocols are provided, which should enable readers to isolate high-quality DNA and RNA from a variety of anaerobic fungal culture media for downstream applications such as next-generation sequencing.Keywords: Anaerobic fungi; Consecutive batch culture; Cultivation; DNA extraction; Genomes; Isolation; Neocallimastigomycota; Next-generation sequencing; RNA extraction; Transcriptomes
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Year: 2018 PMID： 29876808 DOI： 10.1007/978-1-4939-7804-5_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745