Literature DB >> 2987678

Biochemical and immunological characterization of mutant L-M cells with altered levels of dibutyryl cyclic AMP-inducible alkaline phosphatase.

J R Gum, C R Raetz.   

Abstract

Phosphotyrosine-Sepharose 4B was synthesized and used to purify L-cell alkaline phosphatase. Antibodies to this enzyme interacted with the alkaline phosphatase of strains A-1-2 and A-3-3, mutants that express the enzyme constitutively. This and thermal stability studies suggest that these mutants contain the same alkaline phosphatase isozyme as their parent strain.

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Year:  1985        PMID： 2987678      PMCID： PMC366837          DOI： 10.1128/mcb.5.5.1184-1187.1985

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  23 in total

1.  Alkaline phosphatase: affinity chromatography and inhibition by phosphonic acids.

Authors:  M Landt; S C Boltz; L G Butler
Journal:  Biochemistry       Date:  1978-03-07       Impact factor: 3.162

Review 2.  Perspectives on alkaline phosphatase isoenzymes.

Authors:  W H Fishman
Journal:  Am J Med       Date:  1974-05       Impact factor: 4.965

3.  Role of protein glycosylation in the cAMP-mediated induction of alkaline phosphatase in mouse L-cells.

Authors:  G L Firestone; E C Heath
Journal:  J Biol Chem       Date:  1981-02-10       Impact factor: 5.157

4.  Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.

Authors:  J P Chamberlain
Journal:  Anal Biochem       Date:  1979-09-15       Impact factor: 3.365

5.  Biosynthesis of the erythrocyte anion transport protein.

Authors:  W A Braell; H F Lodish
Journal:  J Biol Chem       Date:  1981-11-10       Impact factor: 5.157

6.  Production of antisera with small doses of immunogen: multiple intradermal injections.

Authors:  J L Vaitukaitis
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

7.  Genetic variation in alkaline phosphatase of the house mouse (Mus musculus) with emphasis on a manganese-requiring isozyme.

Authors:  F H Wilcox; L Hirschhorn; B A Taylor; J E Womack; T H Roderick
Journal:  Biochem Genet       Date:  1979-12       Impact factor: 1.890

8.  The cyclic AMP-mediated induction of alkaline phosphatase in mouse L-cells.

Authors:  G L Firestone; E C Heath
Journal:  J Biol Chem       Date:  1981-02-10       Impact factor: 5.157

9.  Phosphotyrosyl-protein phosphatase. Specific inhibition by Zn.

Authors:  D L Brautigan; P Bornstein; B Gallis
Journal:  J Biol Chem       Date:  1981-07-10       Impact factor: 5.157

10.  Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells.

Authors:  K B Seamon; W Padgett; J W Daly
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205
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  2 in total

1.  Cell mutants defective in synthesizing a heparan sulfate proteoglycan with regions of defined monosaccharide sequence.

Authors:  A L De Agostini; H K Lau; C Leone; H Youssoufian; R D Rosenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

2.  Anchoring and degradation of glycolipid-anchored membrane proteins by L929 versus by LM-TK- mouse fibroblasts: implications for anchor biosynthesis.

Authors:  N Singh; D Singleton; A M Tartakoff
Journal:  Mol Cell Biol       Date:  1991-05       Impact factor: 4.272
  2 in total

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